The Peptide Nucleic Acid-Locked Nucleic Acid Polymerase Chain Reaction Clamp-Based Test for Epidermal Growth Factor Receptor Mutations in Bronchoscopic Cytological Specimens of Non-Small Cell Lung Cancer

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概要

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• Objectives: Cytological examination of samples obtained by bronchoscopy is a useful method for establishing the diagnosis of non-small cell lung cancer (NSCLC). However, the utility of a highly sensitive method for the detection of epidermal growth factor receptor (EGFR) mutation in the cytological specimens has not been fully evaluated. Methods: We retrospectively examined the efficacy of the peptide nucleic acid-locked nucleic acid polymerase chain reaction clamp (PNA-LNA PCR clamp) method for detecting EGFR mutations in 122 bronchoscopic cytological specimens from NSCLC patients. Results: Overall, 41 specimens (33.6%) were positive for EGFR mutation. Twenty-nine (39.7%) of 73 specimens obtained by using endobronchial ultrasonography with a guide sheath, 7 (33.3%) of 21 specimens obtained under direct vision by using a conventional bronchoscope, 4 (36.4%) of 11 specimens obtained by using an ultrathin bronchoscope, and 1 (5.9%) of 17 specimens obtained by endobronchial ultrasound-guided transbronchial needle aspiration were positive for EGFR mutation. Furthermore, among 22 resected NSCLC cases, the EGFR mutation status obtained from bronchoscopic materials was consistent with the status obtained from surgical samples, with the exception of one case. Conclusion: The detection of EGFR mutation by subjecting bronchoscopic cytological specimens to a PNA-LNA PCR clamp assay proves useful.

著者

• Oizumi S

  First Department Of Medicine Hokkaido University School Of Medicine

• Oizumi Satoshi

  北海道大学 医学部第一内科学

• Oizumi Satoshi

  First Department Of Medicine Hokkaido University School Of Medicine

• Nishimura M

  First Department Of Medicine Hokkaido University School Of Medicine

• Nakashima M

  Department Of Molecular Immunology And Clinical Immunology Medical Institute Of Bioregulation Kyushu

• Konishi Jun

  First Department Of Medicine Hokkaido University School Of Medicine

• Nakashima M

  Kyushu Univ. Fukuoka Jpn

• Tanaka Tomoaki

  Department Of Respiratory Medicine Saitama Medical University

• Hagiwara Koichi

  Department Of Respiratory Medicine Saitama Medical School

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