Establishment and characterization of amylase-producing lung adenocarcinoma cell line, IMEC-2.
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概要
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Background: Few studies have successfully established an amylase-producing lung cancer cell line or have examined its cytological, biochemical and biological features. Materials and Methods: Cancer cells isolated from pleural effusion using a gradient method were cultivated. Results: Amylase production from the newly established cell line was confirmed by positive staining for α-amylase and increased amylase levels in culture supernatant. Electron microscopy revealed zymogen granule-like structures. Sialylation of salivary-type amylase was confirmed directly from the cell line by examining neuraminidase sensitivity and amylase elution profile under high-performance liquid chromatography. EGFR or KRAS mutation was not found. Conclusions: This cell line offers a useful tool for analyzing the pathogenesis and pathophysiology of amylase-producing lung cancers. Moreover, it might be useful for probing metastasis and invasiveness of lung cancer cells and for developing an early diagnostic method based on sialylated salivary-amylase production.
著者
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Nishimura M
First Department Of Medicine Hokkaido University School Of Medicine
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Yokouchi Hiroshi
First Department Of Medicine Hokkaido University School Of Medicine
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Nakashima M
Department Of Molecular Immunology And Clinical Immunology Medical Institute Of Bioregulation Kyushu
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Itoh Tomoo
School Of Pharmaceutical Sciences Kitasato University
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Dosaka-akita H
Department Of Medical Oncology Hokkaido University Graduate School Of Medicine
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Nakashima M
Kyushu Univ. Fukuoka Jpn
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