Isolation of Ursolic Acid from Apple Peels and Its Specific Efficacy as a Potent Antitumor Agent
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概要
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Dried apple peels were extracted with n-hexane, chloroform, and methanol successively. The portion of the chloroform extract that showed the strongest cytotoxic activity was purified by silica gel chromatography to isolate ursolic acid (UA). The amount of the isolated UA was 0.71% of the dried peels. Normal mouse embryo cells [serum-free mouse embryo (SFME) cells] and tumorigenic human c-Haras- and mouse c-myc-transformed SFME cells [r/m highly metastatic (HM)-SFME-1 cells] were treated with various concentrations of UA (2.5-20μM) to investigate its effects on cell growth. UA at 10μM appeared very effective at suppressing the tumor cell growth, affecting more than 82% of r/m HM-SFME-1 cells, while it inhibited cell growth in only about 7% of SFME cells. Tumorigenic r/m HM-SFME-1 cells were also treated with various concentrations (2.5-10μM) of epidermal growth factor (EGF) or aminoguanidine (AG) in the presence of UA (2.5-10μM). Neither EGF nor AG seemed to have any effect on UA-inhibited cell growth. In the present study, it is revealed that UA could be a very effective and promising agent for antitumor treatments, as it specifically affects tumorigenic cells yet appears to cause very little harm to normal cells.
- 2008-12-01
著者
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HAYASHI Masahiko
The Kitasato Institute
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KOMIYAMA Kanki
The Kitasato Institute
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Komiyama Kanki
The Kitasaro Institute And School Of Pharmaceutical Sciences The Kitasato University
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Hayashi M
The Kitasato Institute
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FUNAYAMA Shinji
Department of Kampo Pharmaceutical Sciences, Nihon Pharmaceutical University
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YAMAGUCHI Hideaki
Department of Material and Life Science, Graduate School of Engineering, Osaka University
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Hayashi Masahiko
The Kitasato Inst.
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Umetsu Hironori
Lab. Of Food Chemistry Dep. Of Life Sciences Junior Coll. Gifu Shotoku Gakuen Univ.
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Noshita Toshiro
Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Aomori University
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Kidachi Yumi
Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Aomori University
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Umetsu Hironori
Laboratory of Food Chemistry, Department of Life Sciences, Junior College, Gifu Shotoku Gakuen Unive
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Ryoyama Kazuo
Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Aomori University
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Ryoyama Kazuo
Graduate School Of Environmental Sciences Aomori Univ.
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Komiyama K
The Kitasato Institute
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Yamaguchi Hideaki
Department Of Material And Life Science Graduate School Of Engineering Osaka University
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Funayama S
Dep. Of Kampo Pharmaceutical Sciences Nihon Pharmaceutical Univ.
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Funayama Shinji
Department Of Bioscience And Biotechnology
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Kamei K
The Kitasato Institute
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Noshita T
Dep. Of Life Sciences Fac. Of Life And Environmental Sciences Prefectural Univ. Of Hiroshima
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Noshita Toshiro
Department Of Bioscience And Biotechnology
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Yamaguchi Hideki
Department Of Applied Physics School Of Engineering The University Of Tokyo
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Kidachi Yumi
Department of Clinical Pharmacy, Aomori University
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RYOYAMA Kazuo
Department of Clinical Pharmacy, Aomori University
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