Differentiation of Serum-Free Mouse Embryo Cells into an Astrocytic Lineage is Associated with the Asymmetric Production of Early Neural, Neuronal and Glial Markers(Molecular and Cell Biology)
スポンサーリンク
概要
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Serum-free mouse embryo (SFME) cells, the astrocyte progenitor cells in the central nervous system (CNS), were exposed to 10 ng/ml leukemia inhibitory factor (LIF) and 10 ng/ml bone morphogenic protein 2 (BMP2) to induce differentiation, and expression of cell-type specific markers. Nestin, a marker of early neural lineage, βIII-tubulin, a marker of neuronal lineage, oligodendrocyte marker O4 (O4), a marker of oligodendrocytic lineage and glial fibrillary acidic protein (GFAP), a marker of astrocytic lineage, were analyzed. Characteristics of SFME cells, as a CNS progenitor, were identified and a possible mechanism, underlying SFME cell specification into an astrocytic lineage upon differentiation, was investigated. These markers were present, both at the initial proliferative phase and after induction of differentiation. GFAP expression increased strongly upon differentiation, while expression of the other markers changed very little. These results indicate that astrocytic differentiation is associated with the asymmetric production of these markers, rather than through induction of astrocytic markers.
- 公益社団法人日本薬学会の論文
- 2008-05-01
著者
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YAMAGUCHI Hideaki
Department of Material and Life Science, Graduate School of Engineering, Osaka University
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Umetsu Hironori
Lab. Of Food Chemistry Dep. Of Life Sciences Junior Coll. Gifu Shotoku Gakuen Univ.
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Kidachi Yumi
Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Aomori University
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Umetsu Hironori
Laboratory of Food Chemistry, Department of Life Sciences, Junior College, Gifu Shotoku Gakuen Unive
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Ryoyama Kazuo
Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Aomori University
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Ryoyama Kazuo
Graduate School Of Environmental Sciences Aomori Univ.
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Yamaguchi Hideaki
Department Of Material And Life Science Graduate School Of Engineering Osaka University
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Kidachi Yumi
Department of Clinical Pharmacy, Aomori University
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RYOYAMA Kazuo
Department of Clinical Pharmacy, Aomori University
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Yamaguchi Hideaki
Department of Industrial Chemistry, Faculty of Engineering, Miyazaki University
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- Differentiation of Serum-Free Mouse Embryo Cells into an Astrocytic Lineage is Associated with the Asymmetric Production of Early Neural, Neuronal and Glial Markers(Molecular and Cell Biology)
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