Expression of Mature Human Interleukin 2 and Its Derivatives in Escherichia coli and Comparison of Their Biological Activity in Vitro(Biological Chemistry)

概要

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A mature human interleukin 2 (hIL-2) and its derivatives that lacked the N-terminal portion were expressed in Escherichia coli under the control of the phase λP_L promoter. They accumulated in the form of insoluble inclusion bodies and accounted for about 30% of the total cellular protein. The mature hIL-2 and its derivatives were further purified and their in vitro biological activity was compared in an IL-2 microassay. The results suggested that the hIL-2 derivatives without the N-terminal three or five amino acids were as active as intact hIL-2 and that those without the N-terminal eight or nine amino acids were less active than the intact form.
社団法人日本農芸化学会の論文
1987-04-23