Cloning and Expression of cDNA for Salmon Prolactin in Escherichia coli(Biological Chemistry)

概要

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cDNA clones which include the coding sequences of chum salmon prolactin (sPRL) have been isolated from a cDNA library prepared from chum salmon pituitary gland poly(A)^+ RNA. A synthetic oligonucleotide probe based on amino acid residues 122 - 127 of sPRL was used as a hybridization probe to select recombinant plasmids carrying the sPRL coding sequences. One of the cDNA clones, which includes the longest cDNA insert, has been sequenced. The cDNA sequence codes for a polypeptide of 210 amino acids, including a putative signal sequence of 23 amino acids. The deduced amino acid sequence of the mature region matched that of the sPRL polypeptide isolated from chum salmon pituitaries (ref. 1). sPRL cDNA was expressed in Escherichia coli under the control of the E. coli trp promoter. sPRL was identified by Western blotting using rabbit antiserum to authentic sPRL.
社団法人日本農芸化学会の論文
1988-04-23