The analysis of N-glycolylneuraminic acid(NeuGc) of hepatoma tissue and K562 cell ferritins using HPLC and mass spectrometry

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概要

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• Ferritin is an iron-storage protein and its serum level is known to increase in the patient of with inflammation and malignant tumor. To further elucidate the difference between ferritins from normal human liver tissue and that of cancer cells, their sialic acids were analyzed. The Western blot analysis and the cytochemical staining using anti-NeuGc antiserum indicated that ferritins from the human hepatocarcinoma tissue and malignant K562 cells contain NeuGc, but that from the normal liver does not. The result was also confirmed by HPLC analysis and MALDI-TOF/MS analysis of sialic acids which were derivatized by the DMB method. It was also shown that the sialic acid content in hepatocarcinoma ferritin was much higher than that in the normal liver ferritin. These results suggest that normal and cancerous liver ferritins are qualitatively and quantitatively different in sialylation. In addition, K562 cells were shown to express NeuGc even if the cells were cultured in serum-free media which lack NeuGc. This is of interest from the current concept that expression of NeuGc in human cells is due to uptake and utilization of exogenous NeuGc. (Communicated by Tamio YAMAKAWA, M.J.A.)

• 日本学士院の論文

著者

• FUKAI Fumio

  Department of Patho-Physiology, Faculty of Pharmaceutical Science, Tokyo University of Science

• Hirano Hisashi

  Yokohama City University Kihara Institute For Biological Research/graduate School Of Integrated Scie

• Fukai Fumio

  Department Of Patho-physilogy Faculty Of Pharmaceutical Sciences Tokyo University Of Science

• Matsuda Haruo

  Department Of Immunobiology Faculty Of Applied Biological Science Hiroshima University

• ASAKAWA Hideo

  Kudanzaka Hospital, Department of Internal Medicine

• SASABE Masataka

  Kudanzaka Hospital, Department of Internal Medicine

• MIYAZAKI Ryuunosuke

  Kudanzaka Hospital, Department of Internal Medicine

• HANADA Kazuki

  Yokohama City University, International Graduate School of Arts and Science

• TAKASAKI Seiichi

  Division of Biochemistry, Institute of Medical Science, University of Tokyo

• Matsuda Haruo

  Department Of Applied Chemistry Faculty Of Engineering Osaka University

• Fukai Fumio

  Department Of Molecular Patho-physiology Faculty Of Pharmaceutical Science

• MATSUDA Haruo

  Department of Microbiology and Hygiene, Faculty of Applied Biological Science, Hiroshima University

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