Three-dimensional Structure of Microbial 2-Hydroxy1-6-oxo-6-phenylhexa-2, 4-dienoic Acid (HPDA) Hydrolase (BphD Enzyme) from Rhodococcus sp. Strain RHA1, in the PCB Degradation Pathway

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概要

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• The three-dimensional structure of an enzyme, 2-hydroxyl-6-oxo-6-phenylhexa-2, 4-dienoic acid (HPDA) hydrolase (conventionally called BphD) from Rhodococcus sp. strain RHA1 has been solved by X-ray crystal structure analysis. This enzyme hydrolyzes one of the highly reactive intermediates, the meta cleavage product of the reaction catalyzed by 2, 3-dihydroxybiphenyl dioxygenase, in the metabolic pathway degrading biphenyl compounds including the notorious environmental pollutant PCBs (polychlorinated biphenyls). By virtue of this and several other enzymes, the biphenyl compounds including PCBs are finally introduced into the tricarboxylic acid cycle. The BphD enzyme is an oligomeric enzyme made up of eight identical subunits each of 285 amino acid residues. The subunit consists of two domains, α/β domain and α domain, between which the active site is located.

• 日本学士院の論文

著者

• YAMADA Akihiro

  Department of Functional Materials Science, Saitama University

• Fukuda Masao

  Department Of Bioengineering Nagaoka University Of Technology

• Fukuda Masao

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• HATTA TAKASHI

  Research Institute of Technology, Okayama University of Science

• Hatta T

  Okayama Univ. Sci. Okayama Jpn

• Hatta Takashi

  Research Institute Of Technology Okayama University Of Science

• MITSUI Yukio

  Department of BioEngineering Nagaoka University of Technology

• Shimada Teruhiko

  Research Institute Of Technology Okayama University Of Science

• Hirose Toru

  Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive

• NANDHAGOPAL Narayanasamy

  Department of BioEngineering, Nagaoka University of Technology

• SENDA Toshiya

  Department of BioEngineering, Nagaoka University of Technology

• Yamada Akihiro

  Department Of Chemistry Graduate School Of Science

• YAMADA AKIHIRO

  Department of Bioengineering, Nagaoka University of Technology

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