Extracellular Production of Klebsiella Pullulanase by Escherichia coli That Carries the Pullulanase Secretion Genes(Microbiology & Fermentation Industry)

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概要

• 論文の詳細を見る

• The pullulanase gene, pulA, and the genes for pullulanase secretion factors (called the pullulanase secretion genes) from Klebsiella aerogenes were cloned into a cosmid vector, pHC79, in Escherichia coll. Four of one thousand clones isolated secreted pullulanase into their culture broth when the cells were grown with pullulan or maltose. One of the four clones produced much pullulanase extracellularly in a short time, with a level of production eightfold that of the maximum production of the wild-type K. aerogenes W70. Deletion analysis of the recombinant plasmid showed that the pullulanase secretion genes and pulA were all in a 22-kb DNA segment derived from the chromosomal DNA of K. aerogenes.

• 社団法人日本農芸化学会の論文
• 1991-06-23

著者

• KIYOHARA Hohzoh

  Department of Applied Chemistry, Faculty of Engineering, Okayama University of Science

• Hatta Takashi

  Central Research Institute, Okayama University of Science

• HATTA TAKASHI

  Research Institute of Technology, Okayama University of Science

• TAKIZAWA NOBORU

  Biotechnology Laboratory, Department of Applied Chemistry, Faculty of Engineering, Okayama Universit

• KIYOHARA HOHZOH

  Biotechnology Laboratory, Department of Applied Chemistry, Faculty of Engineering, Okayama Universit

• Hatta T

  Okayama Univ. Sci. Okayama Jpn

• Hirose Toru

  Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive

• Takizawa N

  Department Of Applied Chemistry Faculty Of Engineering Okayama University Of Science

• Takizawa Noboru

  Department Of Pediatrics Kanazawa University School Of Medicine

• Takizawa Noboru

  Biotechnology Laboratory Department Of Applied Chemistry Faculty Of Engineering Okayama University O

• Kiyohara H

  Department Of Applied Chemistry Faculty Of Engineering Okayama University Of Science

• Kiyohara Hohzoh

  Biotechnology Laboratory Department Of Applied Chemistry Faculty Of Engineering Okayama University O

• NAGAO KAZUTAKA

  Hiruzen Research Institute

• SHIRO Hirohumi

  Biotechnology Laboratory, Department of Applied Chemistry, Faculty of Engineering, Okayama Universit

• Nagao Kazutaka

  Hiruzen Research Institute:okayama University Of Science

• Shiro Hirohumi

  Biotechnology Laboratory Department Of Applied Chemistry Faculty Of Engineering Okayama University O

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