Replication Origin Region of the Chromosome of Alkaliphilic Bacillus halodurans C-125
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概要
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An 18.5-kb DNA fragment containing the oriC region of the chromosome of the alkaliphilic Bacillus halodurans C-125 was obtained by PCR and sequenced. Sixteen open reading frames (ORFs) were identified in this region. A sequencing similarity search using the BSORF database found that ORF1 to 13 all had significant similarities to gene products of Bacillus subtilis. Three other ORFs (ORF14-16) of unknown function were positioned downstream of gyrB instead of rrnO, which is found in the same region in the case of B. subtilis. The ORF organization from gidA to gyrA was the same as that of B. subtilis. The gene organization and the location of the DnaA-box region were also similar to those of the chromosomes of other bacteria, such as Escherichia coli and Pseudomonas putida. There were two DnaA-box clusters (Box-region C and R) with a consensus sequence TTATCCACA on both sides of the dnaA gene but another DnaA box cluster (Box-region L) which is found in the region between thdF and jag in B. subtilis was not found in the corresponding region in the case of alkaliphilic Bacillus halodurans C-125.
- 社団法人日本農芸化学会の論文
- 1999-06-23
著者
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Kawano H
Extremobiosphere Research Center (xbr) Japan Agency For Marine-earth Science And Technology (jamstec
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Nakasone K
School Of Engineering Kinki Univ.
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HORIKOSHI KOKI
DEEP-STAR Group, Japan Marine Science and Technology Ceter
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Horikoshi K
Extremobiosphere Res. Center (xbr) Japan Agency For Marine-earth Sci. And Technol. (jamstec)
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Nakasone Kaoru
School Of Engineering Kinki Univ.
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Takami H
The Deep Star Group Japan Marine Science And Technology Center
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Takami Hideto
Deep-sea Microorganisms Research Group Japan Marine Science And Technology Center
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Masui Noriaki
Deep-sea Microorganisms Research Group Japan Marine Science And Technology Center
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NAKASONE Kaoru
Deep-sea Microorganisms Research Group, Japan Marine Science and Technology Center
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Horikoshi Koki
Deep-sea Microorganisms Research Group Japan Marine Science And Technology Center
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