Changes in Timing and Site of Appearance of a Protease in Xenopus Embryos(Developmental Biology)
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概要
- 論文の詳細を見る
A thiol protease was purified from embryos of Xenopus laevis. This protease has a relative mass (M_r) of 43 k-44 K. Antiserum raised against the protease was used for analysis by Western blotting of proteins from embryos at various stages and from adult liver and heart. A band corresponding to a protein with an M_r of approxmately 44 k was detected in the unfertilized eggs and embryos with the monospecific antiserum. The size of the protein that reacted with the antiserum in a preparation of proteins from the liver of Xenopus laevis was different from that of the proteins from the eggs and embryos (M_r, approximately 70k). Immunohistochemical localization with the antiserum revealed that the protease was more abundant in the animal hemisphere and in the cells derived from the animal hemisphere than in other cells of the embryo. The protease was highly enriched in the cytoplasm of ectodermal cells than the cytoplasm of endodermal cell. When proteins from Xenopus embryos were used as substrate, one protein having Mr of 31 K was mainly digested.
- 社団法人日本動物学会の論文
- 1992-04-15
著者
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Katayama Isao
First Department Of Pathology Saitama Medical School
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IINO Teruhiko
Department of General Studies, College of Humanities and Sciences, Nihon University
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Iino Teruhiko
Department Of General Education College Of Humanities And Science Nihon University
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MIYATA SHOHEI
Laboratory of Research for Biosynthesis and Metabolism, Keio University, School of Medicine
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NISHIBE YASUO
First Department of Pathology, Saitama Medical School
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SENDAI MICHIKO
Department of Chemistry, College of Humanities and Science, Nihon University
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KIHARA HIROZI
Laboratory of Research for Biosynthesis and Metabolism, Keio University, School of Medicine
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Miyata Shohei
Laboratory Of Research For Biosynthesis And Metabolism Keio University School Of Medicine
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Miyata Shohei
Laboratory Of Biochemistry College Of Humanities And Sciences Nihon University
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Kihara Hirozi
Laboratory Of Research For Biosynthesis And Metabolism Keio University School Of Medicine
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Nishibe Yasuo
First Department Of Pathology Saitama Medical School
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Sendai Michiko
Department Of Chemistry College Of Humanities And Science Nihon University
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Iino Teruhiko
Department of Chemistry, Nihon University
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