Effects Of Proteasome Inhibitor (lactacystin) and Cysteine Protease Inhibitor (E-64-d) on Proceesses of Mitosis in Xenopus Embryonic Cells : Biochemistry

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概要

• 論文の詳細を見る

• At least two different protease pathways have been implicated in the degradation that is required to control the eukaryotic cell cycle ; these two pathways center on the activities of ubiquitin/proteasome and cysteine protease. The proteasome inhibitors, lactacystin and AcLLnL and the cysteine protease inhibitor E-64-d were tested for their ability to inhibit the cell cycles of Xenopus embryos. Lactacystin, AcLLnL and E-64-d all caused the complete arrest of the cell cycle. To define the specific cell cycle processes that were affected by the two inhibitors, we performed a cyiologic alanalysis. Inhibition of the cell cycle by lactacystin and E-64-d occurred during prophase and metaphase. The number of cells that arrested in prophase was 1.4-times higher in the E-64-d-treated group than in the control group and the number of arrested cells in the lactacystin-treated group was 1.4-times higher than in the E-64-d-treated group. The number of cells that arrested in metaphase was 3-to-4-times higher in the E-64-d and lacta cystingroups than in the control group. These results indicate that both cysteine protease(s) and proteasomes are involved in the prophase and metaphase stages of cell division.

• 社団法人日本動物学会の論文
• 2002-11-25

著者

• MIYATA SHOHEI

  Laboratory of Research for Biosynthesis and Metabolism, Keio University, School of Medicine

• Miyata Shohei

  Laboratory Of Biochemistry College Of Humanities And Sciences Nihon University

• Sekiguchi Tomohiro

  Laboratory of Biochemistry, College of Humanities and Sciences, Nihon University

• Hosoyama Yoshiyuki

  Laboratory of Biochemistry, College of Humanities and Sciences, Nihon University

• Sekiguchi T

  Laboratory Of Biochemistry College Of Humanities And Sciences Nihon University

• Hosoyama Yoshiyuki

  Laboratory Of Biochemistry College Of Humanities And Sciences Nihon University

関連論文

• Changes in Timing and Site of Appearance of a Protease in Xenopus Embryos(Developmental Biology)
• Neuronal components of the superior and inferior tentacles in the terrestrial slug, Limax marginatus
• Effects Of Proteasome Inhibitor (lactacystin) and Cysteine Protease Inhibitor (E-64-d) on Proceesses of Mitosis in Xenopus Embryonic Cells : Biochemistry
• Sensory Preconditioning in the Terrestrial Mollusk, Limax flavus
• Temporal Analysis of the Retention of a Food-Aversive Conditioning in Limax flavus(Behavior Biology)
• Aversive Conditioning to a Compound Odor Stimulus and Its Components in a Terrestrial Mollusc(Behavior Biology)
• Properties of Wave Propagation in the Oscillatory Neural Network in Limax marginatus(Physiology)
• Contributions of Superior and Inferior Tentacles to Learned Food-Avoidance Behavior in Limax marginatus(Behavior Biology)

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