Purification and Some Properties of Melanoidin Decolorizing Enzymes, P-III and P-IV, from Mycelia of Coriolus versicolor Ps4a
スポンサーリンク
概要
- 論文の詳細を見る
Melanoidin decolorizing enzymes (MDE) were extracted from mycelia of Coriolus versicolor Ps4a and purified by DEAE-Sephadex, DEAE-Sephacel and Sephadex G-200 column chromatographies. MDE of this strain consisted of a main fraction, P-fraction, and a minor fraction, E-fraction, and the P-fraction was composed of at least five enzymes. P-III and P-IV in the P-fraction were picked as typical enzymes of this strain, and their enzymatic properties were investigated. P-III had a molecular weight of 48, 400-50, 000, an optimum pH of 5.5 and an optimum temperature of 30-35°C. P-III required glucose and O2 for the appearance of the activity, and was inhibited by p-CMB, N-BSI, Ag+ and o-phenanthroline. On the other hand, P-IV had a molecular weight of 43, 800-45, 000, an optimum pH of 4.0-4.5 and an optimum temperature of 30-35°C. P-IV could decolorize melanoidin in the absence of glucose and O2, and was inhibited weakly by Ag+, p-CME and N-BSI. P-IV is the enzyme that attacks the melanoidin directly in comparison with P-III which attacks melanoidin indirectly as in the sub-reaction of sugar oxidase. Incidentally, a multiplicative effect between P-III and P-IV for decolorization was observed.
- 社団法人 日本農芸化学会の論文
著者
-
Ohmomo Sadahiro
Institute Of Applied Biochemistry University Of Tsukuba
-
Aoshima Ikuko
Department Of Food And Nutrition Science Faculty Of Home Economics Japan Women's University
-
UEDA KIYOMOTO
Institute of Applied Biochemistry, University of Tsukuba
-
Tozawa Yukiko
Institute Of Applied Biochemistry University Of Tsukuba
-
SAKURADA Noriko
Institute of Applied Biochemistry, University of Tsukuba
-
AOSHIMA Ikuko
Department of Food and Nutrition Science, Faculty of Home Economics, Japan Womens University
関連論文
- Microbial Decolorization of Molasses Waste Water by Mycelia Sterilia D90(Microbiology & Fermentation Industry)
- Screening of Filamentous Fungi Having the Ability to Decolorize Molasses Pigments(Microbiology & Fermentation Industry)
- Decolorization of Molasses Waste Water by a Thermophilic Strain, Aspergillus fumigatus G-2-6(Microbiology & Fermentation Industry)
- Adsorption of Melanoidin to the Mycelia of Aspergillus oryzae Y-2-32(Microbiology & Fermentation Industry)
- A Comparative Study and Phage Typing of Silage-Making Lactobacillus Bacteriophages
- Isolation of Enterocin SE-K4-Encoding Plasmid and a High Enterocin SE-K4 Producing Strain of Enterococcus faecalis K-4
- Isolation and Characterization of Enterocin SE-K4 Produced by Thermophilic Enterococci, Enterococcus Faecalis K-4
- Characterization of a Phage Resistance Plasmid, pLKS, of Silage-Making Lactobacillus plantarum NGRI0101
- Continuous Decolorization of Molasses Waste Water Using Immobilized Lactobacillus hilgardii Cells(Microbiology & Fermentation Industry)
- Screening of Anaerobic Bacteria with the Ability to Decolorize Molasses Melanoidin(Microbiology & Fermentation Industry)
- Transformation of silage-making Lactobacillus strains by electroporation with plasmid vectors
- Biotransformation of D-Xylulose to D-Xylose by Immobilized Enzyme Prepared from Streptomyces flavorirensis
- Biotransformation of d-Arabitol to d-Xylulose by Gluconobacter suboxydans Immobilized within k-Carrageenan
- Continuous Decolorization of Molasses Waste Water with Mycelia of Coriolus versicolor Ps4a
- Purification and Some Properties of Melanoidin Decolorizing Enzymes, P-III and P-IV, from Mycelia of Coriolus versicolor Ps4a
- Detection of Lactic Acid and Amino Acids from Melanoidin Decolorized by Enzymes of Coriolus versicolor Ps4a