Biotransformation of D-Xylulose to D-Xylose by Immobilized Enzyme Prepared from Streptomyces flavorirensis

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概要

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• Screening of Actionmycetes suggested that D-xylose isomerase activity is distributed widely in the genus Streptomyces. Streptomyces flavorirensis showed the strongest D-xylose isomerase activity of the tested strains, and its isomerization yield of D-xylose was 91.5%.For the purpose of enzymatic conversion of D-xylulose to D-xylose, properties of immobilized D-xylose isomerase prepared from S. flavorirensis were investigated. The enzyme activity was stable at high temperature(55℃) and high pH(9.0〜10.5), and was activatetd by Co<2+> and Mg<2+>. These properties were favorable for the continuous conversion of D-xylulose to D-xylose by immobilized D-xylose isomerase. In 8 days of operation at a space velocity of 0.25 h<-1>, 29.4 g of D-xylose was produced from 36.0 g of D-xylulose (yield 81.7%).

• 公益社団法人日本生物工学会の論文
• 1985-08-25

著者

• Ohmomo Sadahiro

  Institute Of Applied Biochemistry University Of Tsukuba

• Ohmomo Sadahiro

  Institute Of Appried Biochemistry University Of Tsukuba ; (present Office)national Grassland Researc

• Ohmomo Sadahiro

  草地試験場

• HASHIHAYATA Takashi

  Center for Basic Research, The Kitasato Institute (TCI)

• ISHIKAWA TAKASHI

  (Present adress)Oriental Yeast Co. Ltd.,

• KUROSAKI YUICHIRO

  (Present adress)Division of Chemistry, Japan Upjohn Research Laboratories

• HASHIZUME TERUTAKA

  (Present adress)Institute of Biochemistry, Japan Merck Banyu Co. Ltd.,

• TOZAWA YUKIKO

  Institute of Applied Biochemistry, University of Tsukuba

• UEDA KIYOMOTO

  Insitute of Applied Biochemistry, University of Tsukuba

• Ueda Kiyomoto

  Insitute Of Applied Biochemistry University Of Tsukuba

• Tozawa Yukiko

  Institute Of Applied Biochemistry University Of Tsukuba

• Hashizume T

  Banyu Tsukuba Research Institute Banyu Pharmaceutical Co. Ltd.

• Hashihayata T

  Center For Basic Research The Kitasato Institute (tci)

• Ishikawa T

  National Aerospace Lab. Tokyo Jpn

• Kurosaki Yuichiro

  (present Adress)division Of Chemistry Japan Upjohn Research Laboratories

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