Identification of the Crude Drug Atractylodes Rhizome (Byaku-jutsu) and Atractylodes Lancea Rhizome (So-jutsu) Using Chloroplast TrnK Sequence as a Molecular Marker
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概要
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Novel methods for molecular authenticaiton of Atractylodes-derived crude drugs (Jutsu) were established based on PCR-restriction fragment length polymorphism (RFLP) and direct sequencing of chloroplast trnK. Two regions inside the chloroplast trnK were selected as molecular markers for identification and discrimination of Atractylodes Rhizome (Byaku-jutsu) and Atractylodes Lancea Rhizome (So-jutsu). The Region 1 fragment (260 bp) amplified from So-jutsu and Wa-byaku-jutsu (Atractylodes Rhizome derived from A. japonica) gave 2 bands of 180 bp and 80 bp on agarose gel electrophoresis after digestion with a restriction endonuclease HinfI, whereas the fragment amplified from Kara-byaku-jutsu (Atractylodes Rhizome derived from A. ovata) remained undigested, which allowed unambiguous identification of Kara-byaku-jutsu. By direct sequencing of Region 2 (436 bp) and comparison of the nucleotide sequence data sets we could not only discriminate Byaku-jutsu and So-jutsu but also identify the original plant species of each crude drug specimen. A simple and reliable protocol for rapid preparation of DNA suitable for PCR from as little as 1 mg of Atractylodes-derived crude drugs was also described.
- 公益社団法人日本薬学会の論文
- 2000-05-01
著者
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KOHDA Hiroshi
Experimental Station of Medicinal Plants, Hiroshima University School of Medicine
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MIZUKAMI Hajime
Faculty of Pharmaceutical Sciences, Nagoya City University
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HIRAOKA Noboru
Niigata College of Pharmacy
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Kohda H
The Experimental Station Of Medicinal Plant Hiroshima University School Of Medicine
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Kohda Hiroshi
Experimental Station Of Medicinal Plants Hiroshima University School Of Medicine
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Hiraoka N
Faculty Of Pharmacy Niigata University Of Pharmacy And Applied Life Sciences
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OKABE Yuka
Faculty of Pharmaceutical Sciences, Nagoya City University
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Mizukami Hajime
Faculty Of Pharmaceutical Sciences Nagoya City University
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Mizukami H
Dep. Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Nagoya City Univ.
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Mizukami Hajime
Faculty Of Pharmaceutical Science Nagoya City University
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Okabe Yuka
Faculty Of Pharmaceutical Sciences Nagoya City University
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Kohda H
Experimental Station Of Medicinal Plants Graduate School Of Biomedical Sciences Hiroshima University
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