Cloning and Characterization of a cDNA Encoding β-Amysin Synthase Involved in Glycyrrhizin and Soyasaponin Biosyntheses in Licorice
スポンサーリンク
概要
- 論文の詳細を見る
An oxidosqualene cyclase cDNA, termed GgbAS1, was isolated from cultured cells of licorice(Glycyrrhiza glabra)by heterologous hybridization with cDNA of Arabidopsis thaliana LUP1 lupeol synthase. The yeast transformed with an expression vector containing the open reading frame of GgbAS1 produced β-amyrin, indicating that GgbAS1 encodes β-amyrin synthase involved in the glycyrrhizin and soyasaponin biosyntheses in licorice. Northern blot analysis showed that the level of β-amyrin synthase mRNA was drastically changed in the cultured licorice cells, whereas the mRNA level of cycloartenol synthase was relatively constant.
- 公益社団法人日本薬学会の論文
- 2001-08-01
著者
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INOUE Kenichiro
Gifu Pharmaceutical University
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Hayashi H
Univ. Tokyo Tokyo Jpn
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Kushiro Tetsuo
Graduate School of Pharmaceutical Sciences, The University of Tokyo
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Shibuya Masaaki
Graduate School of Pharmaceutical Sciences, The University of Tokyo
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Ebizuka Yutaka
Graduate School of Pharmaceutical Sciences, The University of Tokyo
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Hayashi H
Drug Research Division Dainippon Sumitomo Pharma Co. Ltd.
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Hayashi Hideya
Pharmaceuticals And Biotechnology Laboratory Japan Energy Corporation
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Hayashi H
Pharmaceuticals & Biotechnology Laboratory Japan Energy Corporation
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HAYASHI Hiroaki
Gifu Pharmaceutical University
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Inoue K
Kobe Pharmaceutical Univ. Kobe Jpn
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HIRAOKA Noboru
Niigata College of Pharmacy
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IKESHIRO Yasumasa
Niigata College of Pharmacy
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Hiraoka N
Faculty Of Pharmacy Niigata University Of Pharmacy And Applied Life Sciences
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Hiraoka Noboru
Niigata University Of Pharmacy And Applied Life Sciences
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Ebizuka Y
Graduate School Of Pharmaceutical Sciences The Univ. Of Tokyo
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Ebizuka Yutaka
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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Shibuya Masaaki
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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HUANG Pengyu
Gifu Pharmaceutical University
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KIRAKOSYAN Ara
Gifu Pharmaceutical University
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Kushiro Tetsuo
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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渋谷 雅明
Riken Plant Science Center
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Inoue Kenichiro
Gifu Pharmaceutical Univ. Gifu Jpn
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Ikeshiro Yasumasa
Niigata University Of Pharmacy And Applied Life Sciences
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Shibuya Masaaki
Graduate School Of Pharmaceutical Sciences The Univ. Of Tokyo
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