Heterologous Expression and Product Identification of Colletotrichum lagenarium Polyketide Synthase Encoded by the PKS1 Gene Involved in Melanin Biosynthesis
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概要
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The Colletotrichum lagenarium PKS1 gene was expressed in the heterologous fungal host, Aspergillus oryzae, under the starch-inducible α-amylase promoter to identify the direct product of polyketide synthase (PKS) encoded by the PKS1 gene. The main compound produced by an A.oryzae transformant was isolated and characterized to be 1,3,6,8-tetrahydroxynaphthalene (T4HN) as its tetraacetate. Since the PKS1 gene was cloned from C.lagenarium to complement the nonmelanizing albinio mutant, T4HN was assumed to be an initial biosynthetic intermediate, and thus the product of the PKS reaction, but had not been isolated from the fungus. The production of T4HN by the PKS1 transformant unambiguously identified the gene to encode a PKS of pentaketide T4HN. In addition, tetraketide orsellinic acid and pentaketide isocoumarin were isolated, the latter being derived from a pentaketide monocyclic carboxylic acid, as by-products of the PKS1 PKS reaction. Production of the pentaketide carboxylic acid provided insights into the mechanism for the PKS1 polyketide synthase reaction to form T4HN.
- 社団法人日本農芸化学会の論文
- 1999-08-23
著者
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Watanabe Akira
Graduate School of Bioagricultural Sciences, Nagoya University
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Ebizuka Yutaka
Graduate School of Pharmaceutical Sciences, The University of Tokyo
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Fujii I
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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Mori Yumiko
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
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Mori Yuichiro
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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KUBO YASUYUKI
Faculty of Agriculture, Kyoto Prefectural University
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TSUJI GENTO
Faculty of Agriculture, Kyoto Prefectural University
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FUJII ISAO
Graduate School of Pharmaceutical Sciences, The University of Tokyo
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Fujii Isao
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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Tsuji G
Faculty Of Agriculture Kyoto Prefectural University
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Kubo Y
Faculty Of Agriculture Kyoto Prefectural University
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Ardjasa W
Research And Assessment Installation For Agriculture Technology
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Ebizuka Y
Graduate School Of Pharmaceutical Sciences The Univ. Of Tokyo
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Ebizuka Yutaka
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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Mori Yasuhide
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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Watanabe A
Research Division For Development Of Anti-infection Agents Institute Of Development Aging And Cancer
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Watanabe Akira
Graduate School Of Bioagricultural Sciences Nagoya University
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