Molecular Cloning and Functional Expression of cDNAs Encoding Oxidosqualene Cyclases from Costus speciosus
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概要
- 論文の詳細を見る
Costus speciosus produces a large quantity of steroidal glycosides derived from the sole aglycone, diosgenin. Cycloartenol, a product of oxidosqualene cyclase (OSC), is postulated to be a common intermediate for phytosterols of primary metabolism and diosgenin of secondary metabolism, possibly providing a metabolic branch point. Two cDNAs, CSOSC1 and CSOSC2, were cloned from C. speciosus by RT-PCR and cDNA library screening. Both cDNAs encode 759 amino acids with high mutual identity (74%), resembling (>55% identity) the known OSCs. Phylogenetic tree analysis indicated that the gene products occupy distinct positions from those of cycloartenol synthases (CASs) and triterpene synthases from dicotyledonous plants. By functional expression in yeast, CSOSC1 and CSOSC2 were proved to encode a CAS and a multifunctional triterpene synthase, respectively. The present result is the first demonstration of the functional expression of OSCs from monocotyledonous plants.
- 公益社団法人日本薬学会の論文
- 2002-04-01
著者
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市瀬 浩志
武蔵野大薬研
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Ebizuka Yutaka
Graduate School of Pharmaceutical Sciences, The University of Tokyo
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Kawano Noriaki
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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Kawano Noriaki
Medicine And Biosystemic Science Kyushu University Graduate School Of Medical Sciences
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Ebizuka Y
Graduate School Of Pharmaceutical Sciences The Univ. Of Tokyo
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Ebizuka Yutaka
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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ICHINOSE Koji
Graduate School of Pharmaceutical Sciences, The University of Tokyo
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