Differential Expression of Three Oxidosqualene Cyclase mRNAs in Glycyrrhiza glabra(Pharmacognosy)
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概要
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The cultured cells and intact plants of Glycyrrhiza glabra (Fabaceae) produce betulinic acid and oleanane-type triterpene saponins (soyasaponins and glycyrrhizin). To elucidate the regulation of triterpenoid biosynthesis in G. glabra, the CDNA of lupeol synthase, an oxidosqualene cyclase (OSC) responsible for betulinic acid biosynthesis, was cloned, and expression patterns of lupeol synthase and two additional OSCs, β-amyrin synthase and cycloartenol synthase, were compared. The mRNA expression levels of lupeol synthase and β-amyrin synthase were consistent with the accumulation of betulinic acid and oleanane-type triterpene saponins, respectively. The transcript of lupeol synthase was highly expressed in the cultured cells and root nodules. The transcript of β-amyrin synthase, an OSC responsible for oleanane-type triterpene biosynthesis, was highly expressed in the cultured cells, root nodules and germinating seeds, where soyasaponin accumulates, and in the thickened roots where glycyrrhizin accumulates. In the cultured cells, the addition of methyl jasmonate up-regulated β-amyrin synthase mRNA and soyasaponin biosynthesis, but down-regulated lupeol synthase mRNA. Furthermore, the addition of gibberellin A_3 down-regulated β-amyrin synthase mRNA but not lupeol synthase mRNA in the cultured cells. The mRNA levels of cycloartenol synthase, an additional OSC responsible for sterol biosynthesis, in the intact plant and cultured cells were relatively constant in these experiments.
- 公益社団法人日本薬学会の論文
- 2004-07-01
著者
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SUZUKI Masashi
RIKEN Plant Science Center
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INOUE Kenichiro
Gifu Pharmaceutical University
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Hayashi H
Univ. Tokyo Tokyo Jpn
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Shibuya Masaaki
Graduate School of Pharmaceutical Sciences, The University of Tokyo
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Ebizuka Yutaka
Graduate School of Pharmaceutical Sciences, The University of Tokyo
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Hayashi H
Drug Research Division Dainippon Sumitomo Pharma Co. Ltd.
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Hayashi Hideya
Pharmaceuticals And Biotechnology Laboratory Japan Energy Corporation
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Hayashi H
Pharmaceuticals & Biotechnology Laboratory Japan Energy Corporation
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HAYASHI Hiroaki
Gifu Pharmaceutical University
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Inoue K
Kobe Pharmaceutical Univ. Kobe Jpn
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Ebizuka Y
Graduate School Of Pharmaceutical Sciences The Univ. Of Tokyo
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Ebizuka Yutaka
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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Shibuya Masaaki
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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HUANG Pengyu
Gifu Pharmaceutical University
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TAKADA Satoko
Gifu Pharmaceutical University
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OBINATA Megumi
Gifu Pharmaceutical University
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渋谷 雅明
Riken Plant Science Center
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Inoue Kenichiro
Gifu Pharmaceutical Univ. Gifu Jpn
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Shibuya Masaaki
Graduate School Of Pharmaceutical Sciences The Univ. Of Tokyo
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