In Vitro Propagation and Storage of Brugmansia versicolor Lagerheim

概要

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Brugmansia versicolor Lagerheim of the family Solanaceae was propagated through shoot tip culture and shoots were cold-stored in vitro at 5, 10 or 15°C under light or dark condition. All the shoots died after cold storage for 6 months at 5°C irrespective of light condition. When shoots were stored for 12 months at 15°C under light illumination, the best 100% survival rate was obtained. The plants regenerated from shoots stored for 6 or 12 months retained the ability to accumulate scopolamine as much as the control plants which were raised from shoots maintained under normal culture condition without cold-storage. These findings show that slow growth storage of in vitro B. versicolor shoots at 15°C can be used as a germplasm conservation system for short-or medium-term duration without deterioration of the ability to accumulate the secondary metabolites.
日本植物細胞分子生物学会の論文
2004-09-01