Quantitative Analysis of Prion Protein by Immunoblotting
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概要
- 論文の詳細を見る
Transmissible spongiform encephalopathy (TSE) is a neurodegenerative disease characterized by spongiform degeneration and accumulation of an infectious isoform (PrP^<Sc>) of the prion protein in the central nervous system. PrP^<Sc> originates from a ubiquitous cellular prion protein (PrP^C). We attempted to develop an easy method of quantitative analysis of PrP by immunoblotting based on densitometry data for PrP bands in immunoblots. Both PrP^C and PrP^<Sc> yield three bands in immunoblots, and they correspond to PrP molecules carrying two, one, and no Asn-linked sugar chains. We used bovine PrP^C as a model protein in the immunoblotting study. We removed the Asn-linked sugar chains from the PrP molecules with N-glycanase to convert all three glycoforms of PrP into a single band of the deglycosylated form and determined the PrP by densitometry calibrated with recombinant bovine PrP.
- 社団法人日本薬学会の論文
- 2002-06-01
著者
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Horiuchi Motohiro
北海道大学 獣医学研究科プリオン病学
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Sawada Jun-ichi
Department Of Immunobiology Faculty Of Applied Biological Science Hiroshima University
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Yamazaki Takeshi
Division Of Food Additives National Institute Of Health Sciences
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Yamazaki Takeshi
Graduate School Of Science And Technology Niigata University
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Yamazaki Takeshi
National Institute Of Health Sciences
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TANIMURA Akio
Showa Women's University
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KIKUCHI Yutaka
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences
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Tanimura Akio
Science For Living System Graduate School Showa Women's University
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Tanimura Akio
Showa Woman's University
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Takekida Kaori
Showa Woman's University
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Horiuchi Motohiro
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences
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Kakeya Tomoshi
Division of Micro biology and Immunochemistry, National Institute of Health Sciences
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Shinagawa Morikazu
Research Center for Protozoan
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Takatori Kosuke
Division of Micro biology and Immunochemistry, National Institute of Health Sciences
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Tanamoto Ken-ichi
Division of Micro biology and Immunochemistry, National Institute of Health Sciences
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Sawada Jun-ichi
Laboratory of Veterinary Public Health, Department of Veterinary Medicine, Obihiro University of Agr
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Kikuchi Y
National Inst. Of Health Sci. Tokyo Jpn
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Kikuchi Yutaka
Division Of Microbiology National Institute Of Health Sciences
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Horiuchi Motohiro
Division Of Biochemistry And Immunochemistry National Institute Of Health Sciences:research Center F
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Takekida Kaori
Showa Women's University
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Kakeya Tomoshi
Division Of Microbiology National Institute Of Health Sciences
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Kakeya Tomoshi
Department Of Biology Faculty Of Science Okayama University
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Yamazaki T
Division Of Food Additives National Institute Of Health Sciences
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Takatori K
National Institute Of Health Sciences
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Tanimura A
Science For Living System Graduate School Showa Women's University
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Kikuchi Yutaka
Division Of Biochemistry And Immunochemistry National Institute Of Health Sciences:division Of Micro
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Yamada Takashi
National Institute Of Health Sciences (formerly National Institute Of Hygienic Sciences)
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