ELISA Detection of <I>Kudoa septempunctata</I> in Raw <I>Paralichthys olivaceus</I> (Olive Flounder) using a Chicken Anti-<I>Kudoa</I> Antiserum
スポンサーリンク
概要
- 論文の詳細を見る
<I>Kudoa septempunctata</I> is the causative agent of a foodborne disease associated with the consumption of raw <I>Paralichthys olivaceus</I> (olive flounder). Chickens were used to establish specific antibodies against <I>K. septempunctata</I> spores. A specific antiserum, CS#3, raised against sonicated spores, also recognized intact spores. The CS#3 antiserum showed high titers for sonicated and intact <I>K. septempunctata</I> spores and was suitable for both ELISA and immunohistochemical staining. Using homogenated raw olive flounder meat, the ELISA system detected more than 5.0×10<SUP>5</SUP> spores in 1 g of tissue, which was consistent with the number determined by microscopic examination. The preparation of rapid detection kits for <I>K. septempunctata</I> spores in <I>P. olivaceus</I> muscle tissue using immunochromatography with CS#3 antiserum should be useful for preventing the foodborne disease in the field.
著者
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KAWAI Takao
Divisions of Food Microbiology, Osaka Prefectural Institute of Public Health
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Sugita-konishi Yoshiko
Division Of Microbiology National Institute Of Health Sciences
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Ohnishi Takahiro
Division Of Cardiology Yamada Red Cross Hospital
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Kikuchi Yutaka
Division Of Biochemistry And Immunochemistry National Institute Of Health Sciences:division Of Micro
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Yokoyama Hiroshi
Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of
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Furusawa Hiroko
Division of Microbiology, National Institute of Health Sciences
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FUKUDA YUTAKA
Forestry and Fisheries Research, Oita Prefectural Agriculture
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KAWAI TAKAO
Division of Bacteriology, Osaka Prefectural Institute of Public Health
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YOKOYAMA HIROSHI
Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences,University of Tokyo
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