Sensitive Detection of Whole-Genome Differentiation among Closely-Related Species of the Genus Fusarium Using DNA-DNA Hybridization and a Microplate Technique
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概要
- 論文の詳細を見る
We developed a new system for detection of whole-genome differentiation using DNA-DNA hybridization, and tested its sensitivity with three closely-related Fusarium species. We compared DNA-DNA relatedness to nucleotide sequence homologies of five genetic regions between each of five strains of three Fusarium species. DNA-DNA relatedness by our system was 16.2–86.6%. Sequence homologies of 18S rDNA, rDNA cluster region from ITS1 to 28S rDNA, β-tub, EF-1α and lys2 were 100.0, 99.0–100.0, 96.7–100.0, 95.1–99.4, and 94.7–100.0%, respectively. Our system could clearly detect differentiation between closely-related fungal species which have very similar morphological-characteristics, and exhibit little diagnoses in nucleotide sequences. Our results suggest that this system is a good tool for identification and phylogenetic analysis of closely-related fungal species.
著者
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Goto Keiichi
Food Research Laboratories Mitsui Norin Co. Ltd.
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Sugita-konishi Yoshiko
Division Of Microbiology National Institute Of Health Sciences
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Watanabe Maiko
Division Of Rheumatology Department Of Medical Oncology And Immunology Nagoya City University Gradua
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KAMATA Yoichi
Division of Microbiology, National Institute of Health Sciences
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HARA-KUDO Yukiko
Division of Microbiology, National Institute of Health Sciences
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Goto Keiichi
Food Research Institute, Mitsui Norin Co. Ltd.
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WATANABE Maiko
Division of Microbiology, National Institute of Health Sciences
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