Genotyping and Chlorine-Resistance of Methylobacterium aquaticum Isolated from Water Samples in Japan
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概要
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For microbial ecological analysis, 14 strains of Methylobacterium aquaticum isolated from water samples were subjected to clustering analysis on the basis of ribotyping and RAPD-PCR tests. The ribopatterns after digestion with EcoRI obtained from 14 strains of M. aquaticum were used to divide the strains into two groups (Groups I and II) with a similarity of 55%. From the analysis of RAPD patterns using primer 208, the 14 strains were divided into 3 groups (A-C) based on a homology of 45% or greater, and from that using primer 272, there were 4 groups (A-D) based on a homology of 50% or greater. The chlorine resistance (99.9% CT values) of these isolates was also experimentally confirmed, and we attempted to define the connection between chlorine resistance and the geno-cluster. The average CT value of group I was 0.89 mg•min/l and the average of group II was 0.69 mg•min/l. No remarkable differences in the CT values for the groups were found.
- 2011-09-10
著者
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ISHIZAKI NAOTO
School of Life and Environmental Science, Azabu University
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古畑 勝則
Graduate School Of Environmental Health Azabu University
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福山 正文
Graduate School Of Environmental Health Azabu University
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Goto Keiichi
Food Research Laboratories, Mitsui Norin Co., Ltd.
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Goto Keiichi
Food Research Laboratories Mitsui Norin Co. Ltd.
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Fukuyama Masafumi
School Of Environmental Health Azabu University
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Kawakami Yasushi
School Of Life And Environmental Science Azabu University
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Fukuyama Masafumi
School Of Life And Environmental Science Azabu University
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Furuhata Katsunori
School Of Life And Environmental Science Azabu University
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Ishizaki Naoto
School Of Life And Environmental Science Azabu University
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Yoshida Yoshihiro
Food Research Laboratories Mitsui Norin Co. Ltd.
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BANZAI AZUSA
School of Life and Environmental Science, Azabu University
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Banzai Azusa
School Of Life And Environmental Science Azabu University
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Goto Keiichi
Food Research Institute, Mitsui Norin Co. Ltd.
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FURUHATA KATSUNORI
School of Environmental Health
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FUKUYAMA MASAFUMI
School of Environmental Health
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