Gene Expression and the Physiological Role of Transforming Growth Factor-α in the Mouse Pituitary(Endocrinology)
スポンサーリンク
概要
- 論文の詳細を見る
Transforming growth factor-α (TGF-α), a member of the epidermal growth factor (EGF) family, is produced within the mouse anterior pituitaries. However, the cell types of TGF-α-expressing cells and the physiological roles of TGF-α within mouse pituitary glands remain unclear. The aim of the present study was to localize TGF-α mRNA-expressing cells, and to clarify the involvement of TGF-α in estrogen-induced DMA replication in mouse anterior pituitary cells. Northern blot analysis demonstrated TGF-α mRNA expression in adult male and female mouse anterior pituitaries. In situ hybridization analysis of the pituitaries in these mice showed that TGF-α mRNA-expressing cells in the anterior pituitary are round, oval, and medium-sized. TGF-α mRNA was colocalized in most of the growth hormone (GH) mRNA-expressing cells, while only some of the prolactin (PRL) mRNA-expressing cells. DNA replication in the anterior pituitary cells was detected by monitoring the cellular uptake of a thymidine analogue, bromodeoxyuridine (BrdU) in a primary serum-free culture system. Estradiol-17β (E2) and TGF-α treatment increased the number of BrdU-labelled mammotrophs, indicating that E2 and TGF-α treatment stimulates the DNA replication in mammotrophs. Immunoneutralization of TGF-α with anti-TGF-α-antibodies nullified the E2-induced increase in DNA replication. RT-PCR analysis of TGF-α mRNA expression in ovariectomized female mice revealed that E2 increases TGF-α mRNA levels. These results indicate that the TGF-α produced primarily in the somatotrophs mediates the stimulatory effects of estrogen on the DNA replication of pituitary cells in a paracrine or autocrine manner.
- 社団法人日本動物学会の論文
- 2003-01-25
著者
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Takahashi Sumio
岡山大学 理学部生物学科
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Takahashi Sumio
Department Of Applied Physics The National Defense Academy
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Sharma S
Indian Inst. Technol. New Delhi Ind
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Takeuchi Sakae
Department of Biology, Faculty of Science, Okayama University
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Takahashi Sumio
Department Of Applied Physics National Defense Academy
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Masui T
National Institute Of Health Sciences Cell Bank
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Masui Tohru
National Institute For Health Sciences Division Of Mutation And Genetics Japanese Research Resource
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Takeuchi Sakae
Department Of Biology Graduate School Of Natural Science And Technology Okayama University
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Takekida Kaori
Showa Women's University
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Kakeya Tomoshi
Division Of Microbiology National Institute Of Health Sciences
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Kakeya Tomoshi
Department Of Biology Faculty Of Science Okayama University
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Takatori K
National Institute Of Health Sciences
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Takahashi S
Department Of Biology Faculty Of Science Okayama University Tsushima
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Sharma Seema
Department of Biology, Faculty of Science, Okayama University
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Oomizu Souichi
Department of Biology, Faculty of Science, Okayama University
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Oomizu S
Department Of Biology Faculty Of Science Okayama University
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Oomizu Souichi
Department Of Biology Faculty Of Science Okayama University
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Takeuchi S
Department Of Biology Faculty Of Science Okayama University Tsushima
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Takeuchi Sakae
Department Of Biology Faculty Of Science Okayama University
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