Property and Amino Acid Sequence of a Subtilisin Inhibitor from Seeds of Beach Canavalia (Canavalia lineata)
スポンサーリンク
概要
- 論文の詳細を見る
A subtilisin inhibitor was purified from the seeds of Canavalia lineata by ammonium sulfate precipitation, ultrafiltration on a YM-30 membrane, column chromatography on DEAE-Toyopearl and SP-Toyopearl, followed by reverse-phase HPLC. The inhibitor (CLSI-I) is a low molecular weight protein (M_r about 6500)containing no half-cystine residue, and quite stable as to extreme heat and pH treatment. CLSI-I inhibited subtilisin-type serine proteases including S. griseus alkaline protease. The amino acids of CLSI-I were sequenced by manual Edman degradation after enzymatic digestion with Achromobacter lyticus lysyl endopeptidase and Staphylococcus aureus V8 protease. CLSI-I contains 65 amino acid residues and showed a high homology to potato inhibitor I family proteins.
- 社団法人日本農芸化学会の論文
- 1994-11-23
著者
-
Terada Shigeyuki
Department of Chemistry, Faculty of Science, Fukuoka University
-
KATAYAMA Hidekazu
Institute for Protein Research, Osaka University
-
Katayama H
Univ. Tokyo Tokyo Jpn
-
Terada Shigeyuki
Department Of Chemistry Faculty Of Science Fukuoka University
-
Terada S
Faculty Of Science Fukuoka University
-
FUJIMURA Satoshi
Department of Chemistry, Faculty of Science, Fukuoka University
-
KIMOTO Eiji
Department of Chemistry, Faculty of Science, Fukuoka University
-
Katayama Hideki
Department of Chemistry, Faculty of Science, Fukuoka University
-
Soezima Yasuhiro
Laboratory of Biochemistry, Faculty of Engineering, Kyushu Sangyo University
-
Kimoto E
Department Of Chemistry Faculty Of Science Fukuoka University
-
Kimoto Eiji
Department Of Chemistry Faculty Of Science Fukuoka University
-
Soezima Yasuhiro
Laboratory Of Biochemistry Faculty Of Engineering Kyushu Sangyo University
-
Fujimura Satoshi
Department Of Chemistry Faculty Of Science Fukuoka University
関連論文
- Structural Analysis of Two Isoforms of Subunit B in Phospholipase A_2 Inhibitor PLI γ from the Serum of Habu, Trimeresurus flavoviridis
- Effects of a C-terminal Amide Moiety and an Additional Glycine Residue of Crustacean Hyperglycemic Hormone on Its Hormonal Activity
- BACTERIAL EXPRESSION OF A RECOMBINANT CRUSTACEAN HYPERGLYCEMIC HORMONE AND ASSESSMENT OF ITS BIOLOGICAL ACTIVITY
- A Recombinant Molt-inhibiting Hormone of the Kuruma Prawn Has a Similar Secondary Structure to a Native Hormone : Determination of Disulfide Bond Arrangement and Measurements of Circular Dichroism Spectra(Biochemistry & Molecular Biology)
- High-Pressure-Induced Hemolysis of Hereditary Spherocytic Erythrocytes Is Not Suppressed by DIDS Labeling
- In vivo effects of a recombinant molt-inhibiting hormone on molt interval and hemolymph ecdysteroid level in the kuruma prawn, Marsupenaeus japonicus
- MOLECULAR CLONING OF A cDNA ENCODING AN MIH-LIKE PEPTIDE OF THEKKURUMA PRAWN PENAEUS JAPONICUS(Endocrinology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Multigene Family for Bowman-Birk Type Proteinase Inhibitors of Wild Soja and Soybean : The Presence of Two BBI-A Genes and Pseudogenes
- Interaction of Carbodiimide with Human Erythrocytes : Hemolytic Properties Induced by High Pressure, Heating, and Hypotonic Medium
- Efficient Purification of an Antihemorrhagic Factor (HSF) in Serum of Japanese Habu (Trimeresurus flavoviridis)
- High Pressure Sensitizes Murine Erythroleukemia Cells to Caffeine-Induced Premature Mitosis
- Effects of Chemical Modification of Cysteines 201 and 317 of Band 3 on Hemolytic Properties of Human Erythrocytes under Hydrostatic Pressure
- High Pressure Induces G2 Arrest in Murine Erythroleukemia Cells^1
- Effects of Deabetes on Uremic Dyslipidemia
- Design and Synthesis of Arg-Containing Peptides Having Various Secondary Structures and Their Biological Activities
- Preparation and Physicochemical Analysis of 5-Methyl-3,4-Dihydroxytetrone Produced by Oxidative Degradation of Dehydro-L-Ascorbic Acid
- Primary Structure and Autoproteolysis of Brevilysin H6 from the Venom of Gloydius halys brevicaudus
- Purification and Amino Acid Sequence of Brevilysin L6, a Non-Hemorrhagic Metalloprotease from Agkistrodon halys brevicaudus Venom
- Property and Amino Acid Sequence of a Subtilisin Inhibitor from Seeds of Beach Canavalia (Canavalia lineata)
- Amino Acid Sequences of Double-headed Proteinase Inhibitors from the Seeds of Canavalia lineata
- Purification and Characterization of Three Proteinase Inhibitors from Canavalia lineata Seeds
- The Primary Structure of a Concanavalin A-like Lectin from the Seed of Canavalia lineata
- Reinvestigation of the primary structure of concanavalin A from Canavalia ensiformis
- Regional Arterial Stiffness Associated with Ischemic Heart Disease in Type 2 Diabetes Mellitus
- Effect of Atorvastatin of Regional Arterial Stiffness in Patients with Type 2 Diabetes Mellitus
- Pulse Wave Velocity in Lower-Limb Arteries among Diabetic Patients with Peripheral Arterial Disease
- PURIFICATION AND CHARACTERIZATION OF SYNTHETIC POLYPEPTIDES CORRESPONDING TO RIBONUCLEASE T_1 AND ITS ANALOG, [59-TYROSINE] RIBONUCLEASE T_1, BY THE SLID-PHASE METHOD
- SYNTHESIS OF PROTECTED POLYPEPTIDES WITH THE SEQUENCES OF RIBONUCLEASE T_1 AND ITS ANALOG, [59-TYROSINE]RIBONUCLEASE T?1
- Purification and Characterization of Proteinase Inhibitors from Wild Soja (Glycine soja) Seeds(Biochemistry & Molecular Biology)
- Chromatographic separation of brevilysins, metalloproteinases in Chinese Mamushi (Gloydius halys brevicaudus) venom
- Release of Spectrin-Containing Vesicles from Human Erythrocyte Ghosts by Dimyristoylphosphatidylcholine^1
- Excimer Fluorescence of N-(1-Pyrenyl)iodoacetamide-Labeled Spectrin
- Ussuristatin 2, a Novel KGD-Bearing Disintegrin from Agkistrodon ussuriensis Venom
- Novel Function of Antihemorrhagic Factor HSF as an SSP-Binding Protein in Habu (Trimeresurus flavoviridis) Serum
- Characterization of Brevilysin H1, an α-Fibrinogenolytic Metalloproteinase from the Venom of Chinese Snake (Gloydius blomhoffi brevicaudus)
- Isolation and Characterization of a Novel Subunit of Phospholipase A_2 Inhibitor in the Serum of Trimeresurus flavoviridis
- Effects of Anion Transport Inhibitors on Hemolysis of Human Erythrocytes under Hydrostatic Pressure
- HISTOCHEMICAL AND ELECTRON SPIN RESONANCE STUDY OF SERUM METALLOPROTEINS IN DAB HEPATOMA
- Purification and Characterization of a Basic Amino Acid-Specific Peptidase from Seeds of Jack Bean (Canavalia ensiformis)
- Membrane Perturbations of Erythrocyte Ghosts by Spectrin Release
- Caspase Activation in High-Pressure -Induced Apoptosis of Murine Erythroleukemia Cells
- Suppression of High-Pressure-Induced Hemolysis of Human Erythrocytes by Preincubation at 49*C^1
- Cell-Substratum Adhesion Is Suppressed by High Pressure
- High-Pressure-Induced Hemolysis in Papain-Digested Human Erythrocytes Is Suppressed by Cross-Linking of Band 3 via Anti-Band 3 Antibodies
- Purification, Amino Acid Sequence, and cDNA Cloning of Trypsin Inhibitors from Onion (Allium cepa L.) Bulbs(Biochemistry & Molecular Biology)
- INTERACTION BETWEEN ACID MUCOPOLYSACCHARIDES AND BASIC NOXIOUS SUBSTANCES
- Structural Organization and Evolution of a Cluster of Small Serum Protein Genes of Protobothrops Flavoviridis Snake
- Characterization of Brevilysin H2, an α-Fibrinogenolytic Metalloproteinase from the Venom of Chinese Snake (Gloydius blomhoffi brevicaudus)
- Studies of spin-labeled sodium dodecyl sulfate. II Effect of sodium chloride on ESR line width.
- BIOCHEMICAL RESEARCH ON THE INFLUENCE OF PLACENTA PLASMA UPON THE LIVER INJURYCAUSED BY CARBON TETRACHLORIDE
- STUDIES ON THE CHANGE OF COLLAGEN AND MUCOPOLYSACCHARIDE CONTENT IN HEPATIC CIRRHOSIS
- Studies of spin labeled sodium dodecyl sulfate. I. Synthesis and properties.
- Lymphocyte Blast Formation in Rats with DAB-Induced Hepatic Carcinoma
- Antigenic profiles of carcinoma cells of gastrointestinal tract, as detected by immunofluorescence technique.
- ELECTRON SPIN RESONANCE SPECTRA ON THE BLOODS IN DISEASED CONDITIONS
- ON THE NEW METHODS FOR THE STAINING OF MAST CELL
- ISOLATION AND SOME CHARACTERISTICS OF PLACENTAL ALKALINE PHOSPHATASE
- EFFECT OF PLACENTA PLASMA ON THE SULFATE EXCHANGE IN TISSUES
- INFLUENCE OF ASCORBIC ACID UPON THE MUCOPOLYSACCHARIDE FORMATION IN ALGINIC ACIDGRANULOMA OF GUINEA PIGS
- ON THE METHOD TO PREPARE THE PLACENTA-PLASMA
- Preparation and characterization of a 345nm absorbing reductant derived from dehydro-L-ascorbic acid.
- Immunofluorescent staining of gastrointestinal glycoprotein of carcinoma cells in serous effusions.
- EFFECT OF TEMPERATURE ON THE AUTOLYSIS OF COLD-STORED HUMAN PLACENTA
- STUDIES ON THE INTERACTION BETWEEN STEARIC ACID MONOLAYER AND DISSOLVED DYES BY THE SURFACE POTENTIAL MEASUREMENT
- Release of Spectrin-Containing Vesicles from Human Erythrocyte Ghosts by Dimyristoylphosphatidylcholine.
- Primary Structure and Autoproteolysis of Brevilysin H6 from the Venom of Gloydius halys brevicaudus.