Rapid Reconstitution of Tonoplast H^+-Translocating Pyrophosphatase from Cultured Rice Cells into Liposomes
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概要
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We report the rapid and functional reconstitution of H^+-pyrophosphatase (H^+-PPase) from the tonoplast of cultured rice (Oryza sativa L.) cells to proteoliposomes. The CHAPS-solubilized H^+-PPase was incorporated into liposomes by gel-filtration. Both the activities of PP_i-hydrolysis and H^+-pumping were influenced by the lipid-protein ratio and cholesterol.
著者
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Nakamura Yoshiyuki
Molecular Function Laboratory, National Food Research Institute
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Sakakibara Yoshikiyo
Molecular Function Laboratory, National Food Research Institute
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Kobayashi Hideyuki
Molecular Function Laboratory, National Food Research Institute
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Kasamo Kunihiro
Department of Applied Physiology, National Institute of Agrobiological Resources
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Nakamura Yoshiyuki
Chugoku National Agricultural Experiment Station
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Nakamura Yoshiyuki
Molecular Function Laboratory National Food Research Institute Ministry Of Agriculture Farestry And
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Kobayashi Hideyuki
Natl.food Res.insti.
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Sakakibara Yoshikiyo
Molecular Function Laboratory National Food Research Institute
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KASAMO Kunihiro
Research Institute for Bioresources, Okayama University
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Kobayashi Hiroshi
Division Of Biological Regulation And Photobiology National Institute For Basic Biology:graduate Sch
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Nakamura Y
Chugoku National Agricultural Experiment Station
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NAKAMURA Yoshiyuki
Nat. Food Res. Inst.
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KUNIHIRO Kasamo
Natl. Inst. Agrobiol. Resources
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Kasamo K
Research Institute For Bioresources Okayama University
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Nakamura Y
School Of Bioresources Hiroshima Prefectural Univ.
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Kobayashi Hideyuki
Department Of Structural Pathology Institute Of Nephrology
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