Inhibition of Tonoplast and Plasma Membrane H^+-ATPase Activity in Rice (Oryza sativa L.) Culture Cells by Local Anesthetics

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概要

• 論文の詳細を見る

• Tonoplast and plasma membrane vesicles were prepared from rice (Oryza sativa L. var. Yuukara) culture cells with step sucrose gradient (30% and 42.9%, w/v) and/or step dextran T-70 gradient (1% and 8%, w/w) to determine the inhibition of tonoplast and plasma membrane ATP-ases by local anesthetics. The degree to which the anesthetics inhibited these ATPases was of the following order: dibucaine > lidocaine≒tetracaine > procaine≒GABA. Dibucaine ranging in concentration from 0.2 mM to 2 mM inhibited tonoplast ATPase activity more than plasma membrane ATPase, the half inhibitions being 0.8 and 1.1 mM, respectively. The K_m values of tonoplast and plasma membrane ATPases were not affected by dibucaine, but various values were noted for V_<max>. Dibucaine inhibited tonoplast and plasma membrane ATPases solubilized from 0.1% DOC pellet by n-octylglucoside and zwittergent 3-14, respectively. The addition of a phospholipid mixture (asolectin) to solubilized both ATPases had no effect on the inhibition by dibucaine. Thus, local anesthetics may act directly on the ATPase moiety without lipid mediation.

• 日本植物生理学会の論文

著者

• Kasamo Kunihiro

  Department of Applied Physiology, National Institute of Agrobiological Resources

• Kasamo Kunihiro

  Department Of Cell Biology National Institute Of Agrobiological Resources

関連論文

• Rapid Reconstitution of Tonoplast H^+-Translocating Pyrophosphatase from Cultured Rice Cells into Liposomes
• Effect of auxin on Mg^-activated and -inhibited ATPases from mung bean hypocotyls
• Presence of a Na^+-activated ATPase in the Plasma Membrane of the Marine Raphidophycean Heterosigma akashiwo
• Isolation of Subunits of Coupling Factor 1 from Maize and Spinach Chloroplasts and Properties of Combinations of Subunits with ATPase Activity
• Response of Tonoplast and Plasma Membrane ATPases in Chilling-Sensitive and-Insensitive Rice Oryza sativa L.) Culture Cells to Low Temperature
• Inhibition of Tonoplast and Plasma Membrane H^+-ATPase Activity in Rice (Oryza sativa L.) Culture Cells by Local Anesthetics
• Reconstitution and Characterization of H^+-Translocating ATPase from the Plasma Membrane of Phaseolus mungo L. Roots
• Mg^-activated and -inhibited ATPases from mung bean hypocotyls
• In vitro binding of IAA to plasma membrane-rich fractions containing Mg^-activated ATPase from mung bean hypocotyls
• Comparison of Plasma Membrane and Tonoplast H^+-Translocating ATPases in Phaseolus mungo L. Roots

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