Comparison of Plasma Membrane and Tonoplast H^+-Translocating ATPases in Phaseolus mungo L. Roots
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概要
- 論文の詳細を見る
Two membrane fractions were obtained from 16%/26% and 34%/40% interfaces following discontinuous sucrose density gradient centrifugation of a 10,000-80,000 ×g pellet from mung bean (Phaseolus mungo L.) roots. The ATPases in the fractions differed from each other in their sensitivity toward various inhibitors, activation with salts, dependence of activity on pH, and K_m for ATP・Mg^<2+>. Judging from their sensitivity toward inhibitors, the ATPases in the low and high density membranes are considered mainly of tonoplast and plasma membrane origin, respectively. Both ATPases were activated by gramicidin D and nigericin. ATP-induced quenching of quinacrine fluorescence in both fractions required Mg^<2+> and permeant anions such as Cl^- and quenching was collapsed by carbonylcyanide p-trifluoromethoxyphenyl hydrazone. The sensitivities of quenching to the inhibitors were essentially the same as those of ATPase activity in the membranes. These findings suggest the involvement of ATPases in H^+-pumping across a plasma membrane and tonoplast.
- 日本植物生理学会の論文
著者
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Kasamo Kunihiro
Department of Applied Physiology, National Institute of Agrobiological Resources
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Kasamo K
National Institute Of Agrobiological Resoueces
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