Low Temperature-Induced Changes in the Thermotropic Properties and Fatty Acid Composition of the Plasma Membrane and Tonoplast of Cultured Rice (Oryza sativa L.) Cells : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS

スポンサーリンク

概要

• 論文の詳細を見る

• The thermotropic transitions of the plasma membrane and tonoplast from cultured cells of chilling-sensitive (CS) and chilling-insensitive (CI) strains of rice (Oryza sativa L.) were analyzed by monitoring the fluorescence polarization of an embedded fluorophore, I ,6-diphenyl- 1 ,3 ,5-hexatriene (DPH), and their relationship to the degree of unsaturation of fatty acids in phospholipids was examined. Polarization values (P) for the tonoplast from cultured cells of CI rice, in contrast to those from CS rice, exceeded those for the plasma membrane. The values for the tonoplast and plasma membrane from CI cells were somewhat higher than those from CS cell. Thus, the tonoplast of CI cells has the lowest fluidity, while the fluidity of the tonoplast and plasma membrane of CI cells shows greater dependence on temperature. Arrhenius plots of the fluorescence anisotropy parameter {(r_O/r)- 1}^<-1> of DPH in the plasma membrane and tonoplast from CI cells gave a slope that was virtually linear throughout the entire range of temperatures from 50℃ to 10℃. However, in the case of CS cells, a discontinuity was sometimes noted in the curve between 35℃ and 30℃ for tonoplast membranes. The activation energy (E_a) of the anisotropy parameter of DPH in both the plasma membrane and tonoplast from CI cells was greater than that from CS cells. E_a in both cases for CS cells increased with increasing duration of exposure to low temperature (5℃), becoming nearly the same as that for CI cells. . The proportion of unsaturated fatty acids, such as linoleic acid (18:2) and linolenic acid (18:3), in the total phospholipids of the plasma membrane and tonoplast from CI cells was much higher than that from CS cells. In membranes from CS cells, this proportion also increased with increasing duration of exposure to low temperature and reached the value for membranes from CI cells. In particular, in CS cells, the most dramatic change was the change in PE and PC that involved a sharp decrease in levels of 18: I , accompanied by an increase in 18:3. The proportion of unsaturated fatty acids was increased by exposure to low temperature, with an accompanying in-crease in values of E..

• 日本植物生理学会の論文

著者

• Sakaki Takeshi

  National Institute For Environmental Studies 16-2

• Kasamo Kunihiro

  Molecular Function Laboratory Biological Function Division National Food Research Institute

• Kagita Fumiko

  Molecular Function Laboratory National Food Research Institute

• Kasamo K

  National Institute Of Agrobiological Resoueces

• YAMANISHI Hiroyasu

  Molecular Function Laboratory, Biological Function Division, National Food Research Institute

• Yamanishi H

  National Food Research Inst. Ibaraki Jpn

• Yamanishi Hiroyasu

  Molecular Function Laboratory Biological Function Division National Food Research Institute

関連論文

• ^P-NMR Study of the Physiological Conditions in Intact Root Cells of Mung Bean Seedling under Low-Temperature Stress : ENVIRONMENTAL AND STRESS RESPONSES
• Changes in the Cytoplasmic and Vacuolar pH in Intact Cells of Mung Bean Root-Tips under High-NaCl Stress at Different External Concentrations of Ca^ Ions : ENVIRONMENTAL AND STRESS RESPONSES
• Stimulation of the Extrusion of Protons and H^+-ATPase Activities with the Decline in Pyrophosphatase Activity of the Tonoplast in Intact Mung Bean Roots under High-NaCl Stress and Its Relation to External Levels of Ca^ Ions
• ISOLATION AND CHARACTERIZATION OF VACUOLAR H^+ -PYROPHOSPHATASE IN RICE(Oryza sativa L. )
• RECONSTITUTION OF TONOPLAST H^+-PPASE FROM RICE CULTURED CELLS INTO LIPOSOMES
• Reconstitution of Tonoplast H^+-ATPase from Mung Bean (Vigna radiata L.) Hypocotyls in Liposomes
• pH-DEPENDENCES OF MEMBRANE-DIRECTION ANDSIZE OF TONOPLAST VESICLES FROM MUNG BEAN HYPOCOTYLS
• Isolation of Subunits of Coupling Factor 1 from Maize and Spinach Chloroplasts and Properties of Combinations of Subunits with ATPase Activity
• Modulation of the Activity of Purified Tonoplast H^+-ATPase from Mung Bean (Vigna radiata L.) Hypocotyls by Various Lipids
• Low Temperature-Induced Changes in the Thermotropic Properties and Fatty Acid Composition of the Plasma Membrane and Tonoplast of Cultured Rice (Oryza sativa L.) Cells : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS
• Comparison of Plasma Membrane and Tonoplast H^+-Translocating ATPases in Phaseolus mungo L. Roots
• Functional Reconstitution of Plasma Membrane H^+-ATPase from Mung Bean ( Vigna radiata L.) Hypocotyls in Liposomes Prepared with Various Molecular Species of Phospholipids
• Effects of Cerebroside and Cholesterol on the Reconstitution of Tonoplast H^+-ATPase Punfied from Mung Bean(Vigna radiata L.)Hypocotyls in Liposomes : MEMBRANES AND BIOENERGETICS

もっと見る 閉じる

スポンサーリンク