Heterologous Expression, Purification, and Characterization of an α-Mannosidase Belonging to Glycoside Hydrolase Family 99 of Shewanella amazonensis
スポンサーリンク
概要
- 論文の詳細を見る
Shewanella amazonensis α-mannosidase (Sama99), a member of glycoside hydrolase family 99, was expressed in Escherichia coli. The purified Sama99 hydrolyzed pyridylamino (PA)-sugars, Glc1Man9GlcNAc2-PA, and Glc3Man9GlcNAc2-PA, and the product was probably a pyridylamino-decasaccharide in both cases. The mode of action of Sama99 was found to be essentially identical to that of rat endo-α-1,2-mannosidase, but the specificity of Sama99 was low.
- 2011-04-23
著者
-
MATSUO Ichiro
Department of Chemistry and Chemical Biology, Gunma University
-
Matsuo Ichiro
Department Of Chemistry And Chemical Biology Gunma University
-
Tonozuka Takashi
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
-
Nishikawa Atsushi
Department Of Mechanical Science And Bioengineering Graduate School Of Engineering Science Osaka Uni
-
Ito Yukishige
Riken Advanced Science Institute
-
Ito Yukishige
Riken
-
Matsuo Ichiro
Department Of Chemistry & Chemical Biology Gunma University
-
MATSUDA Kana
Department of Applied Biological Science, Tokyo University of Agriculture and Technology
-
KURAKATA Yuma
Department of Applied Biological Science, Tokyo University of Agriculture and Technology
-
MIYAZAKI Takatsugu
Department of Applied Biological Science, Tokyo University of Agriculture and Technology
-
Matsuda Kana
Department Of Applied Biological Science Tokyo University Of Agriculture And Technology
-
Kurakata Yuma
Department Of Applied Biological Science Tokyo University Of Agriculture And Technology
-
Nishikawa Atsushi
Department Of Applied Biological Science Tokyo University Of Agriculture And Technology
-
Miyazaki Takatsugu
Department Of Applied Biological Science Tokyo University Of Agriculture And Technology
関連論文
- 1,2-cis-選択的アミノ糖供与体の開発と抗ピロリ菌活性糖鎖の合成
- Design of an endoscopic solo surgery simulator for quantitative evaluation of the human-machine interface in robotic camera positioning systems
- Detection of Weak Sugar Binding Activity of VIP36 using VIP36-streptavidin Complex and Membrane-based Sugar Chains
- Dinuclear and Tetranuclear Copper(II) Complexes with a Ligand Bearing Phthalocyanine and Schiff-Base Coordination Sites
- Synthesis of the Extracellular Ig Domain I of Emmprin Carrying Chitobiose Unit
- Magnetic navigation system for thoracoscopic surgery: a partial lung resection with transbronchial marking (特集 先進医療に向けたマイクロ技術)
- Structures of Thermoactinomyces vulgaris R-47 α-Amylase II Complexed with Substrate Analogues
- The Deletion of Amino-Terminal Domain in Thermoactinomyces vulgaris R-47 α-Amylases : Effects of domain N on Activity, Specificity, Stability and Dimerization
- Analysis of Catalytic Residues of Thermoactinomyces vulgaris R-47 α-Amylase II (TVA II) by Site-directed Mutagenesis
- Purification, Characterization, and Subsite Affinities of Thermoactinomyces vulgaris R-47 Maltooligosaccharide-metabolizing Enzyme Homologous to Glucoamylases
- Construction of an Efficient Expression System for Aspergillus Isopullulanase in Pichia pastoris, and a Simple Purification Method(Biochemistry & Molecular Biology)
- Stereoselective Total Synthesis of Wheat Flour Ceramide Dihexoside(Organic Chemistry)
- An Efficient Approach to a Lactosamine Synthon for the Synthesis of I-Type Antigens
- Synthesis and properties of polymeric micelles consisting of depsipeptide-poly(ethylene glycol) block copolymer for drug delivery
- The APW Band Calculation of Cs_3Sb
- A Neopullulanase-type α-Amylase Gene from Thermoactinomyces vulgaris R-47
- グリコシル化の高効率立体制御法の開発と細菌由来糖鎖構築への応用
- Cloning and Nucleotide Sequence of the Pullulanase Gene of Thermus thermophilus HB8 and Production of the Enzyme in Escherichia coli(Biochemistry & Molecular Biology)
- PB66 STEREOSELECTIVE GLYCOSYLATION OF NACETYLNEURAMINIC ACID AIDED BY THE STEREOCONTROLLING AUXILIARY
- Wang Resin-Type Linker Containing a Nitro Group for Polymer Support Oligosaccharide Synthesis : Polymer-Supported Glycosyl Donor
- Synthesis of Core-class 2 O-Linked Glycopeptides : a Benzyl-protected Tetrasaccharyl Serine and its Derivative Carrying a Hydrophobic Cholestanyl Group(Organic Chemistry)
- P-109 Mannosylation as Novel Protein Modification : Synthesis and Its Relationship With Diabetes
- Synthesis of Peptide Thioesters Compatible with Solid-Phase Fmoc Methodology
- Molecular Cloning and Characterization of an Enzyme Hydrolyzing p-Nitrophenyl α-D-Glucoside from Bacillus stearothermophilus SA0301
- タンパク質修飾の新しい形 ; C-結合型糖ペプチド
- Identification and Characterization of an Intracellular Lectin, Calnexin, from Aspergillus oryzae Using N-Glycan-Conjugated Beads
- Characterization of Glycoside Hydrolase Family 6 Enzymes from Coprinopsis cinerea
- Pressure Dependence of Energy Gap in CsI Crystal
- On Metallization of CsI under Pressure
- デンプンに作用する酵素とN結合型糖鎖に作用する酵素の構造上の相同性: α-1,X- グルコシド結合をエキソ型で加水分解しβ-グルコースを生成する酵素
- Selective Synthesis of Peptide Bond by Using Formyl-Substituted Phenylthio Ester
- Synthesis of Peptide Thioesters Using a Novel Silyl Linker
- Heterologous Expression, Purification, and Characterization of an α-Mannosidase Belonging to Glycoside Hydrolase Family 99 of Shewanella amazonensis
- 糖質化学の裏庭に漫歩して
- Partial Peptide Sequences from Plasmodium falciparum Enolase as Synthetic Artificial Antigens
- 新しい糖鎖分子プローブ「糖-MTX」の合成と応用
- Silyl Linker-based Approach to the Solid-phase Synthesis of Fmoc Glycopeptide Thioesters(Organic Chemistry)
- Solid-Phase Synthesis of Glycopeptides Using A Novel Silyl Linker
- 収斂的経路による小胞体関連アスパラギン結合型糖鎖の系統的合成
- デンプンに作用する酵素とN結合型糖鎖に作用する酵素の構造上の相同性 : α-1,X-グルコシド結合をエキソ型で加水分解しβ-グルコースを生成する酵素
- Stereocontrolled Synthesis of an Octasaccharide Part of I-Active Glycolipids
- Efficient Synthesis of Fmoc-aminoacyl-N-ethylcysteine, a Key Device for Peptide Thioester Preparation
- Analysis of glycoprotein processing in the endoplasmic reticulum using synthetic oligosaccharides
- Organic Synthesis and Glycobiology
- Synthesis of a Haptasaccharide Hapten Related to an Anomalous Biantennary Glycan Chain of Human Chorionic Gonadotropin of a Patient with a Choriocarcinoma
- P-51 Synthetic study of pseudaminic acid from bacterial glycoprotein
- Excess Resistivity Due to Sb Impurities in Cs Metal
- 凍結反応条件下におけるO-グリコシル化反応の加速と複雑な糖鎖の合成への応用
- A Fluorescent Assay Method for GDP-L-Fuc:N-Acetyl-.BETA.- D-Glucosaminide .ALPHA.1-6Fucosyltransferase Activity, Involving High Performance Liquid Chromatography.
- Studies on the Hydrolyzing Mechanism for Cyclodextrins of Thermoactinomyces vulgaris R-47 .ALPHA.-Amylase 2(TVAII). X-Ray Structure of the Mutant E354A Complexed with .BETA.-Cyclodextrin, and Kinetic Analyses on Cyclodextrins.