Two Components of Cell-bound Isopullulanase from Aspergillus niger ATCC 9642 : Their Purification and Enzymatic Properties
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概要
- 論文の詳細を見る
Cell-bound isopullulanase (pullulan 4-glucanohydrolase: EC 3.2.1.57, IPU) from Aspergillus niger ATCC 9642 [Y. Sakano et al., Denpun Kagaku, 37, 39-41 (1990)] was separated into two active components, IPU F1 (pI=5.0) and IPU F2 (pI=4.9), using a Mono-P HR 5/20 column. The substrate specificity on pullulan and panose, specific activity, optimum pH, pH stability, and susceptibility to certain chemical reagents were similar between IPU F1 and IPU F2. IPU F1 and F2 had an identical N-terminal amino acid sequence, A-V-T-A-D-N-S-Q-L-L-. However, IPU F1 contained more total carbohydrate (15.3%) than IPU F2 (12.4%). SDS-polyacrylamide gel electrophoresis showed that the molecular weight of IPU F1 (71,000) was greater than that of IPU F2 (69,000). After deglycosylation of IPU F1 and F2 with peptide-N-glycosidase F, the molecular weights of IPU F1 and F2 became 59,000.
- 社団法人日本農芸化学会の論文
- 1996-11-23
著者
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Aoki Hiroyoshi
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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Aoki Hiroyoshi
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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Sakano Yoshiyuki
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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Sakano Yoshiyuki
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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PADMAJANTI Anastasia
Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture an
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Padmajanti A
Tokyo Univ. Agriculture And Technol. Fuchu Jpn
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SAKANO Yoshiyuki
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo Noko University
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