Analytical Methods for Quantification of Relative Flying Fish Paste Content in Processed Sea Food (ago-noyaki)
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概要
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Nucleotide sequence and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the 3’-portion of the mitochondrial 16S RNA gene (rDNA) coding sequence was used to authenticate flying fish paste in ago-noyaki. Flying fish paste in ago-noyaki samples was quantified using image analysis of the PCR-RFLP profile. PCR products from standard ago-noyaki samples were digested with AfaI and MunI restriction endonucleases and their electrophoresis patterns were analyzed to produce standard equations for digestion: AfaI digestion: y = 0.0084x + 0.0757, R2 = 0.977 and MunI digestion: y = 0.0091x + 0.0153, R2 = 0.974. This method was then applied to analyze five and two commercially available ago-noyaki and noyaki products, respectively. The results confirmed proper E-mark labeling of ago-noyaki.
- 社団法人 日本食品科学工学会の論文
- 2010-09-01
著者
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NAGASE Mitsutoshi
Shimane Institute of Industrial Technology
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SUGINAKA Katsuaki
Shimane Institute of Industrial Technology
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MORINAGA Tsutomu
Faculty of Life and Environmental Science, Prefectural University of Hiroshima
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Maeta Kazuhiko
Scientific Crime Investigation Laboratory Tottori Police Headquarters
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Morinaga T
Faculty Of Life And Environmental Science Prefectural University Of Hiroshima
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Morinaga T
Hiroshima Univ. Higashi‐hiroshima Jpn
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Aimi Tadanori
Faculty Of Agriculture Tottori University
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Aimi T
Faculty Of Agriculture Tottori University
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Morinaga Tsutomu
Faculty Of Life And Environmental Science Prefectural University Of Hiroshima
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Morinaga Tsutomu
Faculty Of Biological Resources Hiroshima Prefectural University
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Aimi Tadanori
Tottori Univ. Tottori Jpn
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NAGASE Mitsutoshi
Shimane Institute for Industrial Technology
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