P2Y Receptor-Mediated Ca2+ Signaling Increases Human Vascular Endothelial Cell Permeability
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概要
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We investigated the effects of P2-receptor agonists on cell size, intracellular calcium levels ([Ca2+]i), and permeation of FITC-labeled dextran (FD-4) as well as the relationship between these effects in human umbilical vein endothelial cells (HUVEC). FD-4 concentration, cell size, and [Ca2+]i were analyzed by HPLC with fluorescence, phase contrast microscopic imaging, and fluorescent confocal microscopic imaging, respectively. The P2Y1-receptor agonists 2-methylthio ATP (2meS-ATP) and ADP decreased cell size and increased [Ca2+]i in HUVEC. The P2Y2-receptor agonist UTP increased [Ca2+]i, but did not influence cell size. The P2X-receptor agonist α,β-methylene ATP did not induce either response. The decrease in size and increase in [Ca2+]i by 2meS-ATP were blocked by pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS, P2Y1-antagonist), thapsigargin (Ca2+-pump inhibitor), and U73122 (phospholipase C inhibitor). Furthermore, 2meS-ATP (P2Y1-receptor agonist) enhanced permeation of FD-4 through the endothelial cell monolayer. The 2meS-ATP-induced enhancement of the permeation was also prevented by PPADS, thapsigargin, and U73122. These results indicate that activation of P2Y receptors induces a decrease in cell size, an increase in [Ca2+]i, and may participate in facilitating macromolecular permeability in HUVEC.
- 公益社団法人 日本薬理学会の論文
著者
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KUBOTA Yoko
Department of Biopharmaceutics, Nihon Pharmaceutical University
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Kunitomo Masaru
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Mukogawa Women's University
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Nakamura Kazuki
Department Of Applied Chemistry Faculty Of Engineering Shibaura Institute Of Technology
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Hashimoto Michio
Department Of Environmental Physiology Faculty Of Medicine Shimane University
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Kawasaki Kumiko
Department Of Pharmacology School Of Pharmaceutical Sciences Mukogawa Women's University
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Takahashi Koichi
Department Of Biology Faculty Of Science Shinshu University
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TAKAHASHI Koichi
Department of Pharmaceutics, School of Pharmaceutical Sciences, Mukogawa Women's University
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TANAKA Naoko
Department of Gastroenterological Surgery, Transplant, and Surgical Oncology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Nejime Namie
Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's University
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Shinozuka Kazumasa
Department of Pharmacology, School of Pharmaceutical Sciences, Mukogawa Women's University
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Shinozuka Kazumasa
Department of Applied Chemistry, Faculty of Engineering, Yokohama National University
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Kubota Yoko
Department of Pharmacology, School of Pharmaceutical Sciences, Mukogawa Women's University
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Nejime Namie
Department of Pharmacology, School of Pharmaceutical Sciences, Mukogawa Women's University
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Nakamura Kazuki
Department of Pharmacology, School of Pharmaceutical Sciences, Mukogawa Women's University
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Tanaka Naoko
Department of Pharmacology, School of Pharmaceutical Sciences, Mukogawa Women's University
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