The fission yeast RPA21 subunit of RNA polymerase I : an evolutionarily conserved subunit interacting with ribosomal DNA (rDNA) transcription factor Rrn3p for recruitment to rDNA promoter
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概要
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Recruitment of RNA polymerases to the cognate promoter is a key step for the transcription initiation of specific genes in eukaryotes. Recently, RNA polymerase I (pol I) of Saccharomyces cerevisiae was shown to be recruited to the rDNA promoter via interaction between Rrn3p, a conserved transcription factor for rDNA, and A43, a subunit specific to pol I. The question of whether a similar interaction for pol I recruitment is conserved in other eukaryotes remains to be answered. We show here that Schizosaccharomyces pombe rpa21+ encodes a protein of apparent molecular mass 21 kD which shows 36% identity to the A43 subunit of pol I in S. cerevisiae, and that rpa21+ is essential for cell growth. To gain further insight into the functions of RPA21, we isolated a total of 22 temperature-sensitive (ts) mutants of rpa21+ and found that most of the substitutions causing the ts phenotype are clustered in the N-terminal half of RPA21. The ts mutants showed a markedly reduced amount of primary transcripts of rDNA immediately after temperature shift-up. Over-expression of S. pombe rrn3+ in the ts mutants suppressed the growth defect in an allele-specific manner. Therefore, we conclude that S. pombe RPA21 plays a functional role similar to that of A43 in S. cerevisiae and that the mechanism of recruitment of pol I to the rDNA promoter by the interaction of a specific pol I subunit with Rrn3p is evolutionarily conserved.
- 日本遺伝学会の論文
- 2002-06-25
著者
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MURAMATSU Masami
Research Center for Genomic Medicine, Saitama Medical University
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Muramatsu Masami
Department Of Molecular Biology Saitama Medical School
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Muramatsu Masami
Research Center For Genomic Medicine Saitama Medical School
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Nogi Yasuhisa
Department Of Molecular Biology Saitama Medical School
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HISATAKE Koji
Department of Molecular Biology, Saitama Medical School
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IMAZAWA Yukiko
CREST, Japan Science and Technology Corporation Center
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NAKAGAWA Kaori
CREST, Japan Science and Technology Corporation Center
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Hisatake Koji
Department Of Molecular Biology Saitama Medical School
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Imazawa Yukiko
Department Of Molecular Biology Saitama Medical School
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Nakagawa Kaori
Department Of Molecular Biology Saitama Medical School
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