EXPRESSION PATTERN OF THE HOMEOBOX GENE HOX-3.5 DURING MOUSE DEVELOPMENT, AS REVEALED BY A SIMPLIFIED IN SITU HYBRIDIZATION METHOD (<Symposium>In situ Hybridization II. : Application for Development and Nervous System)
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概要
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Improved procedures for a simplified in situ hybridization using paraffin-embedded tissues and riboprobes were described. We found that when tissues were fixed with 4% paraformaldehyde and embedded in paraffin, harsh pretreatments so far employed, such as proteolytic, heat, or HCl treatment each damaging cell morphology to some degree, are not essential to expose mRNAs on the surface of the sections subjected. Typical results are shown for the detection of NGF and EGF mRNAs in mouse submandibular glands. Furthermore, we demonstrated that our method is also applicable to tissues decalcified with 5% ED-TA, as shown in the detection of elastin mRNAs in the calcified tissues in the mouse oral region. We applied our method to determine expression patterns of a new mouse homeobox-containing gone, designated Hox-3.5, Hox-2.1 and Hox-1.3 in embryos. The Hox-3.5 gene is expressed intensively in the rhombencephalon and spinal cord of 12 day embryos, and in the three (at least) cervical ganglion near the occipital bone of 13 day embryos. In the 14 day old embryos, expression of the Hox-3.5 gene is spatially limited at least to the epithelium of both lung and intestine, derived from endoderm. These results suggest that spatial expression ofthe Hox-3.5 gene may play an important role in formation of ectodermal tissues in 12 day embryos and then in generation of the digestive and respiratory tubes from endoderm in 14 day embryos.
- 日本組織細胞化学会の論文
著者
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MURAMATSU Masami
Department of Biochemistry, Saitama Medical School
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NOJI Sumihare
Department of Life Systems, Institute of Technology and Science, The University of Tokushima
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TAKAHASHI Naoki
Department of Clinical Radiology, Graduate School of Medical Sciences, Kyushu University
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NOHNO Tsutomu
Department of Molecular Biology, Kawasaki Medical School
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Yamaai Tomoichiro
Department of Oral Function and Anatomy in Okayama University, Graduate School of Medicine, Dentistr
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Yamaai Tomoichiro
Department Of Cytology And Histology Okayama University Graduate School Of Medicine Dentistry And Ph
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Muramatsu Masami
Research Center For Genomic Medicine Saitama Medical School
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Yamaai Tomoichiro
Department Of Biology Faculty Of Science Chiba University : (present Address) Department Of Anatomy
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Noji S
Department Of Life Systems Institute Of Technology And Science The University Of Tokushima
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Noji Sumihare
Department Of Bioengineering Tokushima University Faculty Of Engineering
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Koyama Eiki
The First Department Of Oral And Maxillcfacial Surgery Okayama University Dental School
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Koyama Eiki
Department Of Orthopaedic Surgery Thomas Jefferson University College Of Medicine
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Muramatsu Masami
Department Of Biochemistry Faculty Of Medicine University Of Tokyo
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Nohno Tsutomu
Department Of Molecular Biology Kawasaki Medical School
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TANIGUCHI SHIGEHIKO
Departmet of Biochemistry, Okayama University Dental School
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Noji Sumihare
Department Of Life Systems Institute Of Technology And Science The University Of Tokushima Graduate
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Taniguchi Shigehiko
Department Of Biochemistry Okayama University Dental School
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Takahashi Naoki
Department Of Cardiology Hyogo Prefectural Amagasaki Hospital
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Takahashi Naoki
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Takahashi Naoki
Department Of Molecular Biology School Of Science Nagoya University
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Nohno Tsutomu
Department of Molecular and Developmental Biology, Kawasaki Medical School
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NOHNO Tsutomu
Department of Pharmacology, Kawasaki Medical School
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