Purification and Cloning of a Ribosomal RNA Gene Fragment from Mouse DNA
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概要
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Newborn mouse DNA was digested with a restriction endonuclease EcoRI and concentrated with respect to ribosomal RNA sequences by an RPC-5 column.DNA fragments of 14-17 kilobases in length, most probably containing promoter region of the ribosomal RNA gene, were used for cloning with λgt WES•λB as a vector using an in vitro packaging technique. Several clones containing 18S rRNA sequences were obtained. One of the clones which was transferred to a plasmid pBR322 (designated as pMrEL-1) was 14 kilobases in length, having only a part of 18S rRNA sequence. These results strongly suggest that this fragment carries a promoter region of the ribosomal RNA gene.
著者
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Mishima Yukio
Department Of Molecular Genetics Graduate School Of Medical And Dental Sciences Niigata University
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Sakai Masaharu
Department Of Biochemistry Hokkaido University School Of Medicine
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Muramatsu Masami
Department Of Biochemistry Faculty Of Medicine University Of Tokyo
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Kataoka Tohru
Department Of Physiology Ii Kobe University School Of Medicine
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HONJO Tasuku
Department of Genetics, Osaka University Medical School
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MURAMATSU Masami
Department of Biochemistry, Cancer Institute , Japanese Foundation for Cancer Research
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SAKAI Masaharu
Department of Biochemistry, Cancer Institute , Japanese Foundation for Cancer Research
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MISHIMA Yukio
Department of Biochemistry, Cancer Institute , Japanese Foundation for Cancer Research
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