Characterization and Identification of a Steroid Receptor-Binding Protein, SRB-RGS(Molecular and Cell Biology)
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概要
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We cloned the cDNA of a novel steroid receptor-binding protein, SRB-RGS, which suppressed the estrogen receptor (ER)α-mediated and other promoter-driven transcriptional activities. This study revealed the interaction between the full-length SRB-RGS and full-length ERα or ERβ by a coimmunoprecipitation assay. The full-length SRB-RGS and full-length ERα interacted in COS-7 cell by a mammalian two-hybrid system. The interaction between intrinsic SRB-RGS and ERs in the nuclear ER extract from the rat uteri was observed by the gelshift assay. These results strongly suggested that SRB-RGS interacts with ERs bound to DNA (estrogen response element) in the nuclei of the cells. SRB-RGS suppressed very efficiently the ERα-, ERβ-, and ERα+ERβ-mediated transcriptional activities. Green fluorescence of enhanced green fluorescence protein (EGFP)-tagged SRB-RGS was localized both in the nucleus and in the cytoplasm. Intrinsic SRB-RGS was immunostained in the nucleus and the cytoplasm of HeLa cells. The putative SRB-RGS deduced from cDNA sequence was identified by the immunostaining and Western blotting by using the anti-SRB-RGS antibody. Overexpression of SRB-RGS induced the cell death in the HeLa cells. The nucleotide sequence of SRB-RGS cDNA that we cloned previously is identical with that of the newly isolated RGS3 cDNA. SRB-RGS could interact with ERs bound DNA in the nuclei of the cells and suppressed the ERs-mediated transcriptional activities.
- 公益社団法人日本薬学会の論文
- 2007-06-01
著者
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Inoue Satoshi
Department of Geriatric Medicine, University of Tokyo
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MURAMATSU Masami
Research Center for Genomic Medicine, Saitama Medical University
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Inoue Satoshi
Department Of Geriatric Medicine The University Of Tokyo
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IKEDA Mitsunori
Department of Dermatology, Kochi Medical School
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MINATOGAWA Yohsuke
Department of Biochemistry, Wakayama Medical College
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Muramatsu Masami
Future Program Research Division Saitama Medical School
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Muramatsu Masami
Research Center For Genomic Medicine Saitama Medical School
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Ikeda Mitsunori
Department Of Biochemistry Kawasaki Medical School
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Minatogawa Yohsuke
Department Of Biochemistry Kawasaki Medical School
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Inoue Satoshi
Department Of Chemistry And Materials Engineering Faculty Of Chemistry Materials And Bioengineering
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Inoue Satoshi
Department of Applied Chemistry, Graduate School of Engineering, Osaka University
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