DEMONSTRATION OF ACTIN AND CYTOKERATIN IN THE NONCILIATED BRONCHIOLAR EPITHELIAL (CLARA) CELL OF RAT
スポンサーリンク
概要
- 論文の詳細を見る
Previously, we perfused the lungs of several mammals (rat, mouse, guinea pig and hamster) with the detergent-containing medium and found that abundant filaments were present in apical caps of nonciliated bronchiolar epithelial (Clara) cells. These filaments were bound to the plasma membrane of the apical cap at one end and ran into the interior of the cytoplasm at the other end. In the present study, we used heavy meromyosin (HMM) and an immunohistochemical method to identify these filaments in rat Clara cell. Most filaments in apical caps bound HMM, but some filaments were not decorated with HMM. To examine the latter filaments, paraffin sections were stained immuno-histochemically utilizing the antibodies against intermediate filament proteins. Apical caps were positive for cytokeratin but were negative for other intermediate filament proteins, vimentin, desmin, neurofilament protein and glial fibrillary acidic protein. These results suggested that filaments in apical cap of nonciliated bronchiolar epithelial (Clara) cells were composed of actin and cytokeratin.
- 日本組織細胞化学会の論文
著者
-
Sasaki J
Department Of Cytology And Histology Okayama University Graduate School Of Medicine And Dentistry
-
Sasaki Junzo
Department Of Cytology And Histology Okayama University Graduate School Of Medicine Dentistry And Ph
-
Sasaki Junzo
Department Of Anatomy Okayama University Medical School
-
NOHNO Tsutomu
Department of Molecular Biology, Kawasaki Medical School
-
Nomura Takako
Department of Anatomy, Okayama university Medical School
-
TANAKA Yukiko
Department of Biochemical Science & Technology, Faculty of Agriculture, Kagoshima University
-
Watanabe Sadahiro
Department Of Anatomy Okayama University Medical School
-
Nomura Takako
Department Of Cytology And Histology Okayama University Graduate School Of Medicine Dentistry And Ph
-
Nomura Takako
Department Of Cytology And Histology Okayama University Graduate School Of Medicine Dentistry And Ph
-
Sasaki J
Okayama Univ. Graduate School Of Medicine And Dentistry
-
佐々木 順造
Department Of Cytology And Histology Okayama University Graduate School Of Medicine Dentistry And Ph
-
Nohno T
Department Of Molecular Biology Kawasaki Medical School
-
KANDA SHIGETO
Department of Anatomy, Okayama University Medical School
-
Nomura Takako
Department Of Anatomy Okayama University Medical School
-
Tanaka Y
Toho Univ. Chiba Jpn
-
Kanda S
Department Of Cytology And Histology Okayama University Graduate School Of Medicine And Dentistry
-
Kanda Shigeto
Department Of Anatomy Okayama University Medical School
-
OTSUKA Nagayasu
Department of Anatomy, Okayama University Medical School
-
Otsuka Nagayasu
Department Of Anatomy Okayama University Medical School
-
Otsuka Nagayasu
Department Of Anatomy Kyoto Prefectural University Of Medicine
関連論文
- Cloning and expression pattern of Xenopus prx-1 (Xprx-1) during embryonic development
- Involvement of Frizzled-10 in Wnt-7a signaling during chick limb development
- Sonic hedgehog signaling during digit pattern duplication after application of recombinant protein and expressing cells
- Involvement of Wnt-5a in chondrogenic pattern formation in the chick limb bud
- LOW-FREQUENCY VIBRATORY SOUND INDUCES NEURITE OUTGROWTH IN PC12M3 CELLS IN WHICH NERVE GROWTH FACTOR-INDUCED NEURITE OUTGROWTH IS IMPAIRED
- COMPARISON OF SPONTANEOUSLY TRANSFORMED XERODERMA PIGMENTOSUM CELLS AND PARTIALLY TRANSFORMED HUMAN DIPLOID CELLS BY X-IRRADIATION
- Expression of Phospholipid Hydroperoxide Glutathione Peroxidase (PHGPx) mRNA in Rat Testes
- Multiple-Labeling of Oligonucleotide Probes for In Situ Hybridization (Recent Progress In Gene Histochemistry)
- VegT, eFGF and Xbra cause overall posteriorization while Xwnt8 causes eye-level restricted posteriorization in synergy with chordin in early Xenopus development
- A Bacterial Strain with Antibacterial and Antioxidant Activities from Tasae, a Burmese Indigenous Alcohol Starter
- Immunization with ACE (Angiotensin Converting Enzyme) Develops Diabetic Changes in the Kidney and Retina in Diabetogenic Rats
- IMMUNOELECTRON MICROSCOPIC LOCALIZATION OF HUMAN T-CELL LEUKEMIA VIRUS TYPE-I ANTIGENS (Immuno-Histochemistry and Cytochemistry)
- DETECTION OF ADULT T-CELL LEUKEMIA-ASSOCIATED ANTIGENS BY DIRECT IMMUNOPEROXIDASE MICROSCOPY WITH Fab'-PEROXIDASE CONJUGATES PREPARED WITH A MALEIMIDE COMPOUND
- Direct immunoelectron microscopic detection of ATLA by a maleimide conjugation method
- Quantification of PERF 15 mRNA in Tissue Sections from Rat Testes
- IA-22 Developing mechanism of follicular atresia in immature rat ovaries : Its connection to the proliferation of ovarian follicular cells, apoptosis, steroid hormone receptor and angiogenesis(THE 46TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY
- Expression of phospholipid hydroperoxide glutathione peroxidase(PHGPx)mRNA in rat testes.
- Immunoelectron Microscopy of the Structural Proteins of Human Immunodeficiency Virus with Monoclonal Antibodies
- MOLECULAR CYTOCHEMISTRY OF NUCLEIC ACIDS BY GENE TECHNOLOGY AND IN SITU HYBRIDIZATION AND THE ADVANCES IN ITS APPLICATION (Molecular Cytochemistry of Nucleic Acids by In Situ Hybridization)
- 排卵と微量元素
- Endothelin-1 Causes Aggregation of Pigment in Teleostean Melanophores
- ___- ___- Detection of Transcription and Translation in Cells Transfected with Molecularly Cloned Retrovirus DNA
- Preparation of and Immunoelectron Microscopy with a Monoclonal Antibody to the Structural Protein of a Retrovirus Produced in a Human Lymphoblastoid Cell Line
- Transcription Complexes of Chromatin Showing a Beads-like Structure of Heterogeneous Ribonucleoprotein Chains
- The Higher Immunoreactivity to ACE (Angiotensin Converting Enzyme) in Patients with Type 2 Diabetes Mellitus than in Non-Diabetic Individuals
- ^F-FDG-PET/CT as an indicator for resection of pulmonary epithelioid hemangioendothelioma
- Clinical significance of the serum IL-2R level and Ga-67 scan findings in making a differential diagnosis between sarcoidosis and non-Hodgkin's lymphoma
- Should mediastinoscopy actually be incorporated into the FDG PET strategy for patients with non-small cell lung carcinoma?
- B-25 Characteristics of inner enamel epithelium in enamel-free area based on the expression of amelogenin mRNA
- Anaerobic dominant flora was reconstructed by synbiotics in an infant with MRSA enteritis
- A Drosophila receptor-type tyrosine kinase (DFR1) acts as a fibroblast growth factor receptor in Xenopus embryos
- EXPRESSION PATTERN OF THE HOMEOBOX GENE HOX-3.5 DURING MOUSE DEVELOPMENT, AS REVEALED BY A SIMPLIFIED IN SITU HYBRIDIZATION METHOD (In situ Hybridization II. : Application for Development and Nervous System)
- SYNTHESIS OF ACTIN IN THE NONCILIATED BRONCHIOLAR EPITHELIAL (CLARA) CELLS OF PERINATAL RATS DEMONSTRATED BY ELECTRON MICROSCOPY AND IN SITU HYBRIDIZATION
- Dynamic aspects of the gene expression in the testis during its atrophy
- Expression of Manganese Suoeroxide Dismutase mRNA in the Rat Adrenal Cortex is Associated with the Occurrence of Mitochondrial Vesicular Cristae
- Variable Number of Tandem Repeat Polymorphism of the Endothelial Nitric Oxide Synthase Gene in Japanese Population
- DETECTION OF HEPATITIS B VIRUS-DNA IN LIVER TISSUES IN CHRONIC TYPE B LIVER DISEASES AND ITS RELEVANCY TO VIRAL ANTIGENS : A STUDY BY IN SITU HYBRIDIZATION USING A BIOTINYLATED PROBE AND STREPTAVIDIN-ALKALINE PHOSPHATASE
- Histochemical and immunohistochemical changes in rat hepatocytes after halothane exposure
- SII-4 Multi-labeling of oligonucleotide probes for in situ hybridization.
- The Expression of Actin mRNA in Megakaryocytes Demonstrated in Paraffin Sections,and Epoxy Resin Semi-Thin and Thin Sections by In Situ Hybridization
- Detection of actin mRNA by ___- ___- hybridization technique
- DEMONSTRATION OF ACTIN AND CYTOKERATIN IN THE NONCILIATED BRONCHIOLAR EPITHELIAL (CLARA) CELL OF RAT
- Cytoskeletal Architecture in the Transitional Epithelial Cells of a Rat Urinary Bladder as Demonstrated by Detergent Perfusion
- THE CYTOSKELETON OF NONCILIATED BRONCHIOLAR EPITHELIAL (CLARA) CELLS.
- Observation of Ehrlich ascites tumor cells by DACM-heavy meromyosin
- Histochemical study of the Mauthner cell
- Histochemical studies of the Mauthner cell
- The site of action of cytochalasin B.
- Embryologic and Histochemical Study of the Conduction System of the Heart in Rats
- POTENTIATION BY PHOSPHATIDYLSERINE OF CALCIUM-DEPENDENT HISTAMINE RELEASE FROM RAT MAST CELLS INDUCED BY CONCANAVALIN A
- Immunoelectron Microscopic Study of Fibronectin on Rat Mast Cells
- Fluorescence Microscopic and Electron Microscopic Observations of the Catecholamine-Granules in the Heart
- ELECTRON MICROSCOPIC STUDY OF THE MOSSY FIBER ENDING IN THE HIPPOCAMPAL FORMATION AFTER DITHIZONE ADMINISTRATION
- A Wnt3a variant participates in chick apical ectodermal ridge formation : Distinct biological activities of Wnt3a splice variants in chick limb development
- Fluorescence and Electron Microscopic Study of Chromaffin Cell in Heart
- Attempts to Identify the Nature on the Specific Granules in Mouse and Rat Atrial Muscle Cells
- Histochemical and Electron Microscopical Studies of the Light and Dark Pyramidal Cells in the Hippocampal Formation
- Gross demonstration of the mammalian Purkinje fiber by a periodic acid-schiff methode and cholinesterase reaction.
- Catecholaminergic Nerve Fibers in the Hippocampal Region
- In vitro Autoradiographical Investigation for Receptor Topography in the Rat Brain
- Ultrastructure of Reduced Nicotinamide Adenine Dinucleotide Phosphate(NADPH)-Diaphorase Positive Neurons in the Cat Central Nervous system
- HISTOCHEMICAL STUDIES ON ZINC OF THE HIPPOCAMPAL FORMATION IN THE MONKEY
- Attempts to Identify the Nature on the Specific Granules in Rat Atrial Muscle Cells.
- Crystallization of Calmodulin Complexed with Bee Venom Melittin
- A STUDY ON THE MOSSY FIBER ENDINGS IN THE HIPPOCAMPAL FORMATION BY ELECTRON MICROSCOPIC AUTORADIOGRAPHY.
- MECHANISM OF HISTAMINE RELEASE BY ALPHA-CHYMOTRYPSIN FROM ISOLATED RAT MAST CELLS
- Interferon-beta, but not tumor necrosis factor-alpha, production in response to poly I:C is maintained despite exhaustive exercise in mice