DETECTION OF ADULT T-CELL LEUKEMIA-ASSOCIATED ANTIGENS BY DIRECT IMMUNOPEROXIDASE MICROSCOPY WITH Fab'-PEROXIDASE CONJUGATES PREPARED WITH A MALEIMIDE COMPOUND
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概要
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Direct immunoperoxidase staining was applied to the detection of adult T-cell leukemia (ATL)-associated antigens (ATLA) in human T-cell lenkemia virus (HTLV-1)-producing MT-2 cells. We successfully conjugated Fab' to peroxidase using a maleimide compound to perform highly sensitive immunohistochemistry with a low background and specific binding. Antisera from three healthy carriers containing antibodies reacting with ATLA were analyzed by Western blotting. ATLA was observed at the light and electron microscopic levels. Positive staining of ATLA was demonstrated in the plasma membrane, endoplasmic reticulum, nuclear nrembrane, and viral particles, but not in the nucleus nor the mitochondria in frozen-sectioned specimens of MT-2 cells fixed with periodate-lysine-paraformaldehyde (PLP) fixative. Control cells (Molt-4) were not stained. The staining in MT-2 cells and HTLV-I represent the distribution of the viral antigens or their precursors.
- 日本組織細胞化学会の論文
著者
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Sumii Hiroshi
Department Of Biochemistry Cancer Institute Okayama University Medical School
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Sumii Hiroshi
岡山大学医学部附属癌源研究施設
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ODA TAKUZO
Department of Biochemistry, Cancer Institute, Okayama University Medical School
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WATANABE SEKIKO
Department of Biochemistry, Cancer Institute, Okayama University Medical School
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Watanabe Sadahiro
Department Of Anatomy Okayama University Medical School
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Oda T
Department Of Biochemistry Cancer Institute Okayama University Medical School
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Oda Takuzo
Department Of Biochemistry Cancer Institute Okayama University Medical School
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Watanabe Sekiko
Department Of Biochemistry Cancer Institute Okayama University Medical School
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Watanabe Sekiko
Department of Anatomy, Okayama University Medical School
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