ELECTRON MICROSCOPIC AUTORADIOGRAPHY OF THE TRANSCRIPTION ON ISOLATED CHROMATIN FIBERS
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概要
- 論文の詳細を見る
In vitro transcription was made on chromatin fibers isolated from rat liver and several types of tumor cells using E. coli RNA polymerase and ^3H-uridine triphosphate-containing substrates. Electron microscopy of the chromatin fibers after the transcription reaction revealed a characteristic structure with RNA-like fibers attached to the chromatin fibers. Electron microscopic autoradiography of these specimens demonstrated silver grains along the RNA-like fibers. This finding provided direct evidence that these fibers were real RNA molecules newly transcribed in vitro. Analysis of the ultrastructure of these transcription complexes revealed that the transcriptionally active regions of the chromatin had extended fibers bound with proteins. Long stretches of free DNA were not found in the active regions.
- 日本組織細胞化学会の論文
著者
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Oda Takuzo
Department Of Biochemistry Cancer Institute Okayama University Medical School
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OMURA SACHIKO
Department of Biochemistry, Cancer Institute, Okayama University Medical School
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Omura Sachiko
Department Of Biochemistry Cancer Institute Okayama University Medical School
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- THE MOLECULAR CYTOCHEMISTRY OF CHROMATIN
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