SYNTHESIS OF ACTIN IN THE NONCILIATED BRONCHIOLAR EPITHELIAL (CLARA) CELLS OF PERINATAL RATS DEMONSTRATED BY ELECTRON MICROSCOPY AND IN SITU HYBRIDIZATION
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概要
- 論文の詳細を見る
Previously, we found abundant filaments consisting of actin and cytokeratin in nonciliated bronchiolar epithelial (Clara) cells by treating the cells with detergent-containing medium. In this study, the presence of the filaments and the expression of actin mRNA in Clara cells of perinatal rats were investigated by electron microscopy and in situ hybridization, respectively. Clara cells in prematal rat were characterized by the presence of glycogen and the absence of electron-dense granules. Detergent treatment did not clarify the presence of filaments in prenatal cells. After birth, there was a rapid loss of glycogen and electron-dense secretory granules appeared in the cytoplasm of Clara cells. Filaments could be recognized by detergent treatment. By the 3rd postnatal day, some Clara cells showed features similar to the cells in adult rats. To investigate whether these filaments demonstrated by detergent treatment were synthesized during the postnatal period, the localization of actin mRNA was observed using an in situ hybridization technique. Signals showing the presence of actin mRNA were observed in many cells at every stage of development. In the prenatal period, the signals were distributed diffusely in the epithelial cells of the bronchioles, but in the postnatal period, they appeared in Clara cells selectively. These results show that the actin filaments observed in the apical caps of Clara cells are synthesized during the postnatal period when Clara cells are thought to become mature.
- 日本組織細胞化学会の論文
著者
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NOJI Sumihare
Department of Life Systems, Institute of Technology and Science, The University of Tokushima
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Sasaki J
Department Of Cytology And Histology Okayama University Graduate School Of Medicine And Dentistry
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Sasaki Junzo
Department Of Cytology And Histology Okayama University Graduate School Of Medicine Dentistry And Ph
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Sasaki Junzo
Department Of Anatomy Okayama University Medical School
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NOHNO Tsutomu
Department of Molecular Biology, Kawasaki Medical School
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Nomura Takako
Department of Anatomy, Okayama university Medical School
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Yamaai Tomoichiro
Department Of Cytology And Histology Okayama University Graduate School Of Medicine Dentistry And Ph
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Watanabe Sadahiro
Department Of Anatomy Okayama University Medical School
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Nomura Takako
Department Of Cytology And Histology Okayama University Graduate School Of Medicine Dentistry And Ph
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Nomura Takako
Department Of Cytology And Histology Okayama University Graduate School Of Medicine Dentistry And Ph
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Noji S
Department Of Life Systems Institute Of Technology And Science The University Of Tokushima
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Noji Sumihare
Department Of Bioengineering Tokushima University Faculty Of Engineering
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Sasaki J
Okayama Univ. Graduate School Of Medicine And Dentistry
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Koyama Eiki
The First Department Of Oral And Maxillcfacial Surgery Okayama University Dental School
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佐々木 順造
Department Of Cytology And Histology Okayama University Graduate School Of Medicine Dentistry And Ph
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Nohno T
Department Of Molecular Biology Kawasaki Medical School
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TANIGUCHI SHIGEHIKO
Departmet of Biochemistry, Okayama University Dental School
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KANDA SHIGETO
Department of Anatomy, Okayama University Medical School
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YAMAAI TOMOICHIRO
The First Department of Oral Anatomy, Okayama University Dental School
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Nomura Takako
Department Of Anatomy Okayama University Medical School
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Noji Sumihare
Department Of Life Systems Institute Of Technology And Science The University Of Tokushima Graduate
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Taniguchi Shigehiko
Department Of Biochemistry Okayama University Dental School
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Kanda S
Department Of Cytology And Histology Okayama University Graduate School Of Medicine And Dentistry
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Kanda Shigeto
Department Of Anatomy Okayama University Medical School
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