Dynamic aspects of the gene expression in the testis during its atrophy

概要

論文の詳細を見る
Although reactive oxygen species (ROS) underlie the pathogenesis of various diseases, they also play critical roles in the regulation of the redox balance required for physiological processes. Although possible involvement of ROS in the pathogenesis of di(2-ethylhexyl) phthalate (DEHP)-induced atrophy of rat testis has been suggested, the molecular mechanism of this phenomena has not been elucidated. We previously investigated the gene expression for phospholipid hydroperoxide glutathione peroxidase (PHGPx) in rat testes by in situ hybridization. PHGPx mRNA was expressed stage-specifically during spermatogenesis in adult rats. Its expression first appeared in stage VII pachytene spermatocytes and markedly in spermatids between steps 7 and 12. Mitochondrial PHGPx plays an important role in the regulation of the mitochondria-dependent pathway of apoptosis. Hypophysectomy decreases PHGPx level in the testis and induces the degeneration of germ cells in rat seminiferous tubules around stage VII. The research focused on changes in the gene expression in the testis in animals that received either hypophysectomy (10-week rats, 5.5 days after the operation) or DEHP administration (35-day rats, 2g/kg); both conditions induced the degeneration of germ cells. The expression of several genes. including lipid-binding protein (TLBP), markedly decreased in the testis of both groups as analyzed by cDNA microarray. However, ISH study did not show a decrease proportional to the results of the gene expression obtained by cDNA microarray. Based on these observations, the usefulness of cDNA microarray in detecting critical molecules in the pathogenesis of testis atrophy will be discussed.
日本組織細胞化学会の論文

Dynamic aspects of the gene expression in the testis during its atrophy

スポンサーリンク

概要

著者

関連論文

スポンサーリンク