Metabolism of administered (2RS)-naringenin in flavonoidproducingcultured cells of Sophora flavescens

概要

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Cultured cells of Sophora flavescens produce (2S)-naringenin-derived prenylated flavanone sophoraflavanone Gand liquiritigenin-derived trifolirhizin 6′-O-malonate. The regulation of flavonoid biosynthesis was examined by analyzingthe metabolites produced in the cultured cells fed (2RS)-naringenin. The amount of sophoraflavanone G in cells fed 0.1 or0.3 mM (2RS)-naringenin was two-fold that in control cells, although the conversion ratio was only 5 to 10% of theadministered (2S)-naringenin. On the other hand, (2R)-naringenin, which does not occur naturally, was efficiently convertedinto its 4′,7-di-O-β-D-glucoside. (2S)-Naringenin prenylation activity was higher at the logarithmic growth stage. The cellsfed (2RS)-naringenin at a lower concentration (below 0.1 mM), accumulated sophoraflavanone G as the main prenylatedflavanone. In contrast, cells fed 0.3 mM (2RS)-naringenin accumulated 8-prenylnaringenin and leachianone G, intermediatesof sophoraflavanone G in large amounts. Accumulation of trifolirhizin 6′-O-malonate was suppressed by the addition ofnaringenin.
日本植物細胞分子生物学会の論文
2004-12-01