Anthocyanin production in adventitious root cultures of Raphanus sativus L. cv. Peking Koushin

概要

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The adventitious roots of Raphanus sativus L. cv. Peking Koushin were established by culturing root segments of in vitro seedlings in 1/2 Murashige and Skoog (MS) liquid medium supplemented with 0.5 mg/l IBA. The adventitious roots cultured in 1/2 MS liquid medium supplemented with 0.5 mg/l IBA produced anthocyanin in the dark. When cultured under the 14 h/day light condition, the adventitious roots cultured with 0.5 mg/l IBA produced more anthocyanin than those cultured with 0.1 or 0.5 mg/l NAA. The acid hydrolysis of the extract prepared from the adventitious roots revealed that the main anthocyanidin was pelargonidin. In addition, the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity of the adventitious root extract was comparable to that of roots of the intact plant grown in the field.
日本植物細胞分子生物学会の論文
2004-12-01