Production of transgenic chickens from purified primordial germ cells infected with a lentiviral vector(GENETICS, MOLECULAR BIOLOGY, AND GENE EXPRESSION)
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概要
- 論文の詳細を見る
Replication-defective retroviral or lentiviral vectors have been used for the production of transgenic animals. Chicken primordial germ cells (PGCs) are the precursors for ova and spermatozoa. Here, we describe the production of transgenic chickens via a germline transmission system using PGCs infected with a replication-defective lentiviral vector. PGCs were sorted with a fluorescence-activated cell sorter based on the expression of stage-specific embryonic antigen-1 from 2.5- and 5.5-day embryos. PGCs from both stages of embryo were infected with a lentiviral vector at a similar efficiency in vitro. PGCs were then transferred into the bloodstream of 2.5-day recipient embryos. The efficiency with which the PGCs were delivered and settled in the gonads was lower for PGCs from 5.5-day embryos than those from 2.5-day embryos when a limited number of PGCs was transferred, while the difference was not obvious upon the transfer of increased number of cells. Using a high number of 5.5-day PGCs infected with a lentiviral vector. transgenic chimeras (G_0) with an acceptable efficiency for germline transmission were obtained. G_0 female chickens produced transgenic progeny (G_1) with higher efficiency compared to G_0 male chickens. In G_1 transgenic chickens obtained by this method, enhanced green fluorescent protein was effectively expressed under the control of the actin promoter.
著者
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Hattori Yuki
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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IIJIMA SHINJI
Department of Chemical Engineering, Faculty of Engineering, Nagoya University
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Iijima S
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Iijima Shinji
Department Of Biotechnology School Of Engineering Naogya University
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Iijima Shinji
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Iijima S
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Iijima Shinji
Department Of Chemical Engineering Nagoya University
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Motono Makoto
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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Yamada Yuki
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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Nakagawa Ryo
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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Nishijima Ken-ichi
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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Nishijima Ken-ichi
Nagoya University
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Morshed Mahboob
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Nakagawa Ryo
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Motono Makoto
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Nishijima Ken-ichi
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Nishijima Ken-ichi
Department Of Applied Biological Chemistry The University Of Tokyo:(present Address)department Of Bl
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Yamada Yuki
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Motono Makoto
Department Of Biotechnology Graduate School Of Engineering Nagoya University:(present Office)departm
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Iijima Shinji
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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Nishijima Kenichi
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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IIJIMA Shinji
Department of Agricultural Chemistry, The University of Tokyo
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YAMADA Yuki
Department of Applied Chemistry, Faculty of Engineering, Tokai University
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