Molecular Cloning a Thermostable β-Glucosidase Gene from a Thermophilic Anaerobe NA10 and Its High Expression in Escherichia coli
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概要
- 論文の詳細を見る
A gene encoding β-glucosidase was cloned from a thermophilic anaerobe strain NA10 into Escherichia coli. The 4.6 kb of HindIII fragment was shown to direct the synthesis of β-glucosidase. A homologous DNA sequence was found to be present in strain NA10 by Southern blot analysis. The β-glucosidase was purified from the recombinant E. coli. The molecular weight of the enzyme was estimated to be 50.1 kDal using SDS-poly-acrylamide gel electrophoresis. The enzyme was heat stable; the optimum pH and temperature were 5.5 and 85℃, respectively. The enzyme could hydrolyse low molecular weight β-1,3 and β-1,4 glucans. The β-glucosidase was produced in the cytosol of the recombinant E. coli (7-20% of total protein), without fusion to any E. coli promoter sequences.
- 公益社団法人日本生物工学会の論文
- 1994-02-25
著者
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Kondo Osamu
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Honda Hiroyuki
Department Of Bioengineering Tokyo Institute Of Technology
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Uozumi N
Nagoya Univ. Nagoya Jpn
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Iijima Shinji
Department Of Biotechnology School Of Engineering Naogya University
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Iijima Shinji
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Sota Hiroyuki
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Sota Hiroyuki
Department Of Applied Chemistry Gifu University
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Kobayashi Takeshi
Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University
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PATTHRA ARUNWANICH
Department of Biotechnology, Faculty of Engineering, Nagoya University
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ARUNWANICH Patthra
Department of Biotechnology, Faculty of Engineering, Nagoya University
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Uozumi Nobuyuki
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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KURITA OSAMU
Department of Biotechnology, Faculty of Engineering, Nagoya University
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Patthra Arunwanich
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Arunwanich Patthra
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Honda Hiroyuki
Department Of Anesthesia Niigata City General Hospital
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IIJIMA Shinji
Department of Agricultural Chemistry, The University of Tokyo
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