Production of Peroxidase with Horseradish Hairy Root Cells in a Two Step Culture System
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概要
- 論文の詳細を見る
Horseradish hairy root cells transformed by a soil bacterium Agrobacterium rhizogenes had peroxidase(POD)activity comparable to that of the original plant root tubers. Tp enhance POD production by the hairy root culture, various additives to the medium were tested including casein hydroysates and plant extracts.Polypepton addition had a significant effect on the growth and POD production; at low concentrations(below1g/l)the growth was stimulated, while at high concentrations(3-10g/l), POD activity in the cells was enhanced in spite of a low growth rate. Therefore, the hairy roots were at first cultured in the medium with 1g/l Polypepton, and then 5g/l Polypepton was added to enhance intracellular POD activity. POD activity in this two step culture system was 5.4 times higher than that in conventional culture in Polypepton-free medium.
- 社団法人日本生物工学会の論文
- 1989-01-25
著者
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Taya Masahito
Department Of Chemical Engineering Faculty Engineering Science Osaka University
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Kondo Osamu
Department of Pediatrics, National Cardiovascular Center
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Kondo O
Sapporo Medical Univ. Sapporo Jpn
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Kondo O
Tohoku Univ. School Of Medicine Sendai Jpn
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Kondo Osamu
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Kondo O
Nagoya Univ. Nagoya Jpn
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NOMURA Ryuji
Department of Condensed Matter Physics, Tokyo Institute of Technology
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Taya Masahito
Department Of Chemical Engineering
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Nomura Ryuji
Department Of Anatomy Fujita Health University School Of Medicine
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Nomura Ryuji
Department Of Chemical Engineering
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Taya Masahito
Department Of Biotechnology Graduate School Of Engineering Osaka University
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YOYAMA AKIHIKO
Department of Chemical Engineering
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MATUI CHIAKI
Department of Agronomy, Nagoya University
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KOBAYASHI TEKESHI
Department of Chemical Engineering
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Matsui C
Department Of Agronomy Nagoya University
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Taya M
(present Address)faculty Of Engineering Science Osaka University
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Matui Chiaki
Department Of Agronomy Nagoya University
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Kondo Osamu
Department Of Anatomy Sapporo Medical College
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