Evaluation of Attachment and Growth of Anchorage-Dependent Cells on Culture Surfaces with Type I Collagen Coating

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The effects of coating the culture surface with bovine type I collagen on the culture properties of anchorage-dependent cells were investigated. When human fibroblasts were cultured on a surface coated with collagen at 5.8×10^<-3>mg/cm^2, cell attachment and subsequent cell growth were both enhanced compared to the culture on an uncoated surface. The degrees of cell attachment and growth enhancement were numerically characterized using the time constant of cell adhesion (τ) and doubling time (t_d) as kinetic parameters. These parameters applied to cultures of human keratinocytes and rabbit chondrocytes allowed the effects of collagen coating on the respective culture properties of both types of cells to be evaluated. In addition, the relative parameters R_τ and R_t_d (defined as the ratios of the τ and t_d values at a given collagen concentration against those without collagen coating, respectively) were employed to estimate the effects of collagen based on a standardized criterion. Similar R_τ and R_t_d profiles were obtained for collagen concentrations raiging from 5.8×10^<-13> to 5.8×10^<-3> mg/cm^2, whether the cells were fibroblasts, keratinocytes or chondrocytes. It was also revealed that coating the surface with collagen at a concentration over 5.8×10^<-7> mg/cm^2 led to reductions in both the R_τ and R_t_d values, i.e.the promotion of cell attachment and growth, in the culture of each type of cells examined.
社団法人日本生物工学会の論文
2001-10-25

Evaluation of Attachment and Growth of Anchorage-Dependent Cells on Culture Surfaces with Type I Collagen Coating

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