Characterization of Pak-Bung Green Hairy Roots Cultivated under Light Irradiation
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概要
- 論文の詳細を見る
Hairy roots of pak-bung turned green when cultured under continuous light exposure, and maintained their branched root morphology with the formation of chlorophyll and the development of a thylakoid membrane system in the cells. Green hairy roots cultivated under light exhibited increased root growth and enhanced activities of superoxide dismutase (SOD) and peroxidase (POD). A light intensity of 11.1 W/m^2 using white fluorescent lamps was found to support good root proliferation and relatively high activities of SOD and POD. In cultures of green hairy roots at a light intensity of 11.1 W/m^2,root growth, total SOD activity and total POD activity obtained on culture day 21 were 8.1 g-dry root weight/dm^3,18×10^4 U/dm^3 and 40×a10^3 U/dm^3,respectively. In dark culture, the corresponding values obtained on culture day 21 were 3.8 g-dry root weight/dm^3,3.0×10^4 U/dm^3 and 9.5×10^3 U/dm^3,respectively. It is concluded that exposure of the green hairy root culture to light caused an increased number of growing points at the root tip meristems due to the frequent branching of lateral roots.
- 社団法人日本生物工学会の論文
- 1994-07-25
著者
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SATO Hitoshi
Department of Clinical and Molecular Pharmacokinetics/Pharmacodynamics, School of Pharmaceutical Sci
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紀ノ岡 正博
阪大院・工・生命先端
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紀ノ岡 正博
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ. 2-1 Yamadaoka Suita Osaka 565-0871
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Kino-oka Masahiro
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Taya Masahito
Department Of Chemical Engineering Faculty Engineering Science Osaka University
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TONE Setsuji
Department of Chemical Engineering, Faculty Engineering Science, Osaka University
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Sato Hitoshi
Department Of Cardiovascular Medicine Tohoku University Graduate School Of Medicine
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Tone Setsuji
Department Of Chemical Engineering Faculty Of Engineering Science Osaka University
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Taya Masahito
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Sato Hitoshi
Department Of Chemical Engineering Faculty Of Engineering Science Osaka University
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Sato Hitoshi
Department Of Cardiology Juntendo University School Of Medicine
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